Baseline

Factors influencing the uptake of PCBs and DDTs in red mullet (Mullus barbatus) from Pagassitikos Gulf, central Greece

In seasonal breeders, such as amphibians, testicular functions depend on complex processes that change according to seasonality, including Leydig cell (LC) differentiation and lipid-dependent steroidogenesis, extracellular proteins remodeling and actin-dependent cellular dynamics. Speculating that fat bodies (FB) could support some of these processes in L. catesbeianus, we evaluated bilaterally the FB weights, correlating them to testicular parameters such as weight, testosterone (T) immunoexpression, mast cells (MC) number, vascularization and structural proteins. In an attempt to better understand the testicular asymmetry in amphibians, correlations between these different testicular parameters were also established. Right testes (RT), left testes (LT) and associated FB of bullfrogs were weighed, and testes were processed for light and transmission electron microscopy. Collagen content (COL) and MC number were quantified. T and actin immunoexpressions and vascular areas were measured. Statistical analyses and Pearson’s correlation were performed.

The LT and its associated FB were heavier than the right ones, and showed intense T and actin immunoexpressions, numerous lipid-rich LC, and greater MC number, COL and vascularization than the RT. Positive correlations were detected between: a) FB and testis weights, b) T immunoexpression and testis and FB weights, c) T and actin immunoexpressions and COL. Otherwise, MC number was inversely correlated to T immunoexpression and COL. In right and left sides, the proportional correlation between T immunoexpression and FB weight suggests that FB-stored lipid amount depends on the steroidogenic demand of its associated testis. Thus, the asymmetry in the testes and FB may be associated, at least in part, to the LC steroidogenic activity, which tends to be more intense in LT than in RT. The results also point to a role of COL and mast cells in the LC differentiation and steroidogenesis. Actin was also greater in LT and correlated with T immunoexpression, indicating that the amount of this structural protein depends on androgenic control. Therefore, the testicular asymmetry in bullfrogs seems to be associated to different morphofunctional processes occurring, bilaterally, at different intensities. In this case, there is a tendency of LT, in association with its FB, to be more active than RT. The findings highlight the FB-testis interplay for the comprehension of reproduction in amphibians.

The existence of Organochlorine pesticides (OCP's) and trace metals in tropical aquatic environments may cause a severe threat on account of their ubiquitous nature and long persistence. Monitoring of such contaminants has assumed immense significance in recent times. The present study assessed the OCP's and trace metal contamination in the surface sediments of the Vembanad wetland system in India. Various pollution indices were calculated to determine the sediment quality of the system and were also compared with uncontaminated sediments. Geo-accumulation index for Mercury varied from moderately to extremely polluted. The mean concentration of pesticide residues and trace metals in the sediments of Vembanad is found to be higher than in other aquatic systems in India. The situation demands further studies which focus on the potential bioaccumulation of OCPs and metals in the benthic food chain, succeeding biota and their impact on human consumption as the system supports a huge population for their livelihood. The Present study provides a baseline information on the spatial distribution of priority pollutants along with ecological risk status of Vembanad estuarine system (VES).

Mercury (Hg) is one of the main chemicals currently altering Mediterranean ecosystems. Red mullet (Mullus barbatus and M. surmuletus) have been widely used as quantitative bio-indicators of chemical contamination. In this study, we reassess the ability of these species to be used as efficient bio-indicators of Hg contamination by monitoring during 18 months Hg concentrations in muscle tissue of mullet sampled from 5 French Mediterranean coastal areas. Mean concentrations ranged between 0.23 and 0.78 μg g⁻¹ dry mass for both species. Values were consistent with expected contamination patterns of all sites except Corsica. Results confirmed that red mullets are efficient bio-indicators of Hg contamination. Nevertheless, the observed variability in Hg concentrations calls for caution regarding the period and the sample size. Attention should be paid to environmental and biologic specificities of each studied site, as they can alter the bioaccumulation of Hg, and lead to inferences about environmental Hg concentrations.

This paper documents the accumulation and emerging levels of Persistent organic pollutants (POPs) such as polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) found in the tissues of the migrating salmon species Oncorhynchus tshawytscha (Chinook salmon) in the Chilean Patagonia area. Even though salmons are not native to the southern hemisphere, reports indicate that Chinook salmon in the last few years have performed natural free-living style cycling, returning to rivers in the southern Patagonia area. Our study seeks to determine the presence and levels of PCB’s and PBDE’s in wild Chinook salmon in the northern part of the Chilean Patagonia, analyzing their relation with physiological parameters. Fish were sampled at the end of their entire life cycle when they returned to two principal rivers in the Aysen region in southern Chile. A number of fish (12) were sacrificed in situ and muscle samples were taken for PCBs measurements (sum 44 congeners) by gas chromatography electron capture detector (GC-ECD) and a number of PBDEs congeners, by gas chromatography mass spectrometry with negative chemical ionization detection (GC–MS NCI). Observed levels and patterns were characterized by concentrations of these POPs for PCBs ranging between 78 and 25.5 ng g⁻¹ wet weight and for PBDEs ranging between 272 and 1046 pg g⁻¹ wet weight, respectively. These ranges are among same levels reported in this same species in the northern hemisphere.

In order to assess the contamination of edible marine organisms in Quanzhou and Xinghua Bays, Fujian Province, China, concentrations of organochlorine pesticides (hexachlorocyclohexane isomers or HCHs and dichlorodiphenyltrichloroethane isomers/derivatives or DDTs) and heavy metals (Cu, Pb, Zn, As, Cr, Cd, Ni and Hg) in 13 species collected from different sites in the bays were analyzed by GC-ECD and ICP-MS. The concentration of the sum of DDTs exceeded that the sum of HCHs in the samples. Most of the organisms showed higher levels of DDTs than the first level criterion (10 ng/g) for marine biological quality in China (GB 18421-2001) but conformed to the first level criterion (20 ng/g) for HCHs. The estimated mean daily uptake of pesticides was below the level for minimal risk to the consumers. The levels of Cd, As and Pb in most organisms from both bays were markedly elevated and above the recommended legal limits for human consumption. Our results showed the need for routine monitoring of marine species contamination to ensure public and environmental health.

Detailed analyses of persistent organic pollutants (POPs) such as hexachlorocyclohexane (HCH) isomers (HCHs), dichlorodiphenyltrichloro ethane (DDT) and its metabolites (DDTs), hexachlorobenzene (HCB) and congeners of polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs) in surface sediments from the coastal estuarine environment of West Bengal, northeast India allowed the evaluation of the contamination status, distribution and possible pollution sources. HCH, DDT, HCB, PCB and PAH were identified compounds in all the samples, whereas the concentrations of chlorinated pesticides (trans-eptachlorepoxide, dieldrin, endrin, metaoxychlor and mirex) were below the detection limits and were not of great concern. The pesticides did demonstrate markedly different distributions reflecting different agricultural and domestic usage in the region. The range of concentrations of HCH, HCB, DDT and PCBs in the sediments were 0.11–0.40, <0.05–0.98, 0.18–1.93 and 0.18–2.33 ng/g dry wt, respectively. Overall elevated levels of HCB, DDT and PCB were recorded at Babughat, a very closely located site of the metropolitan megacity Calcutta. Fluoranthene (flu) and pyrene (py), the dominant 4-ring PAHs, also showed an abrupt elevated concentrations at Babughat with values of 214 and 144 ng/g dry wt, respectively. Among the isomers and metabolites of HCH, DDT and PCB, α-HCH, pp′-DDT and PCB ₁₀₁, PCB ₁₁₈, PCB₁₅₃ and PCB ₁₃₈ were found to be dominant. High ratios of metabolites of DDT to ΣDDTs reveal recent use of DDT in this coastal environment. Fluoranthene, pyrene, 1,2-benzo(a)anthracene and chrysene formed the dominant congeners out of 19 identified PAHs. The sources of contamination are closely related to human activities such as domestic and industrial discharge, automobile exhausts, street runoff, slum sewage, agricultural chemicals and soil erosion due to deforestation as well as atmospheric transport. This study is compared to other coastal and estuarine environments in India and abroad. The baseline data can be used for regular ecological monitoring, considering the industrial and agricultural growth around this important estuarine ecosystem.