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SV40 DNA: Quantitative conversion of closed circular to open circular form by an ethidium bromide-restricted endonuclease

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Abstract

Closed circular molecules of SV40 DNA can be quantitatively converted to the open circular form by digestion with endonucleases in the presence of ethidium bromide. Under these conditions almost no molecules were digested beyond the open circular form, whereas in the absence of the dye the DNA was completely fragmented.

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Cited by (26)

  • The path of the DNA along the dimer interface of topoisomerase II

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    Citation Excerpt :

    Plasmid pKS6 was converted into a pair of 3.6-kb singly linked circles by Tn3 resolvase (22). Selective nicking of the circle containing the unique BamHI site was carried out by controlled digestion with BamHI in the presence of ethidium bromide (23). The singly nicked dimeric catenane was purified by density gradient centrifugation (24).

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This work was generously supported by the Chanock Foundation.

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