Optimizing follicular dendritic cell isolation by discontinuous gradient centrifugation and use of the magnetic cell sorter (MACS)☆
References (26)
- et al.
Isolation of follicular dendritic cells from human tonsils and adenoids. V. Effect on lymphocyte proliferation and differentiation
Immunol. Lett.
(1986) - et al.
Isolation of follicular dendritic cells from human tonsils and adenoids
J. Immunol. Methods
(1984) - et al.
Proliferation of germinal center B lymphocytes in vitro by direct membrane contact with follicular dendritic cells
Immunobiology
(1991) - et al.
Follicular dendritic cells contain a unique gene repertoire demonstrated by single-cell polymerase chain reaction
Blood
(1991) - et al.
Cytokines produced in lymph follicles
Immunol. Lett.
(1989) - et al.
Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes)
J. Histochem. Cytochem.
(1984) - et al.
Influence de la cellule folliculaire dendritique sur Ia survie des lymphocytes in vitro
C.R. Soc. Biol.
(1986) - et al.
Human dendritic reticulum cells of lymphoid follicles: Their antigenic profile and their identification as multinucleated giant cells
Virchows Arch.
(1983) - et al.
The use of avidin-biotin interaction in immunoenzymatic techniques
J. Histochem. Cytochem.
(1979) - et al.
Isolation and properties of spleen follicular dendritic cells
Double immunofluorescence analysis of lymphoid tissue during HIV-1 infection
The follicular dendritic cell: Its role in antigen presentation in the generation of immunological memory
Immunol. Rev.
Adhesion of human B cells to follicular dendritic cells involves both the lymphocyte function-associated antigen 1/intercellular adhesion molecule 1 and very late antigen 4/vascular cell adhesion molecule 1 pathways
J. Exp. Med.
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miRNA expression profiling divides follicular dendritic cell sarcomas into two groups, related to fibroblasts and myopericytomas or Castleman's disease
2016, European Journal of CancerCitation Excerpt :Other studies, however, could not corroborate this finding [31]. In previous studies [32] and own attempts, isolation of primary FDCs from tonsils proved to be very sophisticated and not yielding isolates with an appropriate purity. Although no differences were found between the two FDC sarcoma groups on morphologic grounds, PDPN, which was previously described to be expressed in FDCs and FDC sarcomas [20,21], was significantly more frequently expressed in FDC sarcoma group I. PDPN is expressed in cancer-associated fibroblasts [33] and marginal reticular cells [34] and may even be a therapeutic target for FDC sarcoma group I patients in future [35].
Isolation of functionally active murine follicular dendritic cells
2006, Journal of Immunological MethodsImmunomagnetic separation reagents as markers in electron microscopy
2002, Journal of Immunological MethodsFunctional effects of TNF and lymphotoxin α1β2 on FDC-like cells
2000, Cellular ImmunologyRole of follicular dendritic cells in the apoptosis of germinal center B cells
2000, Immunology Letters
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Part of this work was supported by the Bundesministerium für Forschung und Technologie (Germany) by grant FKZ BGA III-006-89/FVP 6 (P.R. and K.T.-R.).