Short communicationAnalysis of United Kingdom purchased spices for aflatoxins using an immunoaffinity column clean-up procedure followed by high-performance liquid chroma
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2011, Journal of Dairy ScienceCitation Excerpt :For each matrix, separation of aflatoxins was performed on the same reversed-phase C18 (Nucleodur column, 150 mm × 4.6 mm) maintained at 40°C and fitted with a C18 guard column supplied by Macherey-Nagel (Düren, Germany) by using an isocratic mobile phase: acetonitrile:methanol:water:acetic acid (17.5:17.5:64:1), acetonitrile:water (25:75), and isopropanol-acetonitrile-acetic acid-water (10:10:2:78) set at 1 mL/min flow rate for urine, milk, and feces, respectively. For feces extracts, detection of AFB1 was improved by postcolumn derivatization with pyridinium bromide perbromide at 0.05 mg/mL (Garner et al., 1993). Intake, BW, and milk production were used as generic indicators of the effect of aflatoxin and YCW treatments on ewes.
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