Differentiation of neurons and myocytes in cell cultures made from Drosophila gastrulae

doi:10.1016/0014-4827(71)90321-1

Experimental Cell Research

Volume 69, Issue 1, November 1971, Pages 161-173

https://doi.org/10.1016/0014-4827(71)90321-1 Get rights and content

Abstract

Single Drosophila melanogaster embryos of known developmental stages were dissociated and their cells cultured in vitro. Nearly all cells from blastulae disintegrated in vitro. Certain cells taken from embryos at the time gastrulation began or later, could differentiate in vitro to form neurons or pulsating myocytes by 28 h in vitro. Observations made by electron microscopy indicated that early gastrula cells are unspecialized in gross morphology and ultrastructure. Axon generation by neurons and cell elongation by myocytes therefore represented gross morphological differentiations in vitro. Cells with ultrastructures characteristic of neurons and myocytes were found after seven days in culture. These findings confirmed the neuron and myocyte cell ultrastructure in vitro. The pulsations of myocytes indicated their sarcoplasm differentiated to a functioning level.

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^☆: This work was supported by NIH grants AI05038 and NS09330 (R.L.S.), CAO8791 (R.L.T.), National Science Foundation grant GB6634 to Seymour Benzer, and a Ministère des Affaires Etrangères, France, Fellowship (N. A.), and by the Poultry Industry Research Fellowship in honor of W. E. McKibben (R. L. S.), and the Zachary Pitts Research Fellowship (R. L. T.).

¹: Present address: Laboratoire de Zoologie Expérimentale, Faculté des Sciences, 33 Talence, France.

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Experimental Cell Research

Abstract

References (18)

Devel biol

Devel biol

Exptl cell res

Compt rend acad sci Paris

Genetika

Growth

Science

Cited by (80)

PTPN21/Pez Is a Novel and Evolutionarily Conserved Key Regulator of Inflammation In Vivo

Cells or Tissues in Course of Differentiation

Primary Cell Cultures of Drosophila Embryonic Cells

Chapter 3 Mapping and Manipulating Neural Circuits in the Fly Brain

Sub-cellular Ca<sup>2+</sup> dynamics affected by voltage- and Ca<sup>2+</sup>-gated K<sup>+</sup> channels: Regulation of the soma-growth cone disparity and the quiescent state in Drosophila neurons

Selected Methods for the Anatomical Study of Drosophila Embryonic and Larval Neuromuscular Junctions

Differentiation of neurons and myocytes in cell cultures made from Drosophila gastrulae☆

Abstract

Devel biol

Devel biol

Exptl cell res

Compt rend acad sci Paris

Genetika

Growth

Science