Full paperEvidence that the voltage-dependent component in the fertilization process is contributed by the sperm☆
References (19)
- et al.
A fast block to polyspermy in frogs mediated by changes in the membrane potential
Dev. Biol
(1980) - et al.
A long-lasting electrically mediated block, due to the egg membrane hyperpolarization at fertilization, ensures physiological monospermy in eggs of the crab Maia squinado
Dev. Biol
(1989) - et al.
Mastoporan, a peptide toxin from wasp venom, mimics receptors by activating GTP-binding regulatory proteins (G-proteins)
J. Biol. Chem
(1988) An electrically mediated block to polyspermy in the primitive urodele Hynobius nebulosus and phylogenetic comparison with other amphibians
Dev. Biol
(1989)- et al.
Studies of the voltage-dependent polyspermy block using cross-species fertilization of amphibians
Dev. Biol
(1983) - et al.
Voltage-dependent trans-bilayer orientation of melittin
J. Biol. Chem
(1982) The fertilization potential is not necessary for the block to polyspermy or the activation of development in the medaka egg
Dev. Biol
(1980)- et al.
The catecholamine binding site of the β-adrenergic receptor is formed by juxtaposed membrane-spanning domains
J. Biol. Chem
(1988) - et al.
Identification and sequence of a binding site peptide of the β2-adrenergic receptor
Biochem
(1988)
Cited by (33)
The need of MMP-2 on the sperm surface for Xenopus fertilization: Its role in a fast electrical block to polyspermy
2014, Mechanisms of DevelopmentCitation Excerpt :We previously proposed that a positive molecule on the sperm membrane is involved in sensing the voltage of the egg membrane at fertilization (Iwao and Jaffe, 1989). The level of voltage to prevent fertilization is dependent on the sperm species in the fertilization of amphibians (Iwao and Jaffe, 1989; Jaffe et al., 1983) and in marine invertebrates (Jaffe et al., 1982). In the present study, we showed that pre-treatment of the sperm membrane with negatively charged GM1 or the antibody against the positively charged HPX domain allows for sperm to fertilize eggs clamped at 0 mV, at which level fertilization never occurs in insemination with untreated sperm.
Defending the Zygote: Search for the Ancestral Animal Block to Polyspermy
2005, Current Topics in Developmental BiologyCitation Excerpt :Voltage‐clamp studies of other anuran eggs, which do not exhibit such specializations for fusion, have shown that the efficacy of depolarization is instead dependent on its maximum amplitude. For example, the peak Vm achieved by one species is optimized to block supernumerary fusion of only conspecific sperm, and is not sufficient to repel less sensitive heterospecific sperm who require a higher voltage potential to be deterred or who are simply insensitive to membrane voltage potentials (Iwao and Jaffe, 1989; Jaffe et al., 1983a). To establish a timely fast electrical block requires a rapid signaling cascade that likely originates from the sperm itself (Iwao and Jaffe, 1989).
Fertilization signalling and protein-tyrosine kinases
2000, Comparative Biochemistry and Physiology - B Biochemistry and Molecular BiologyCitation Excerpt :This membrane potential is believed to be involved in the fast block to polyspermy, since sperm cannot fertilize eggs when the egg membrane voltage is maintained at larger than 0 mV (Iwao and Jaffe, 1989). It has been shown that this may be due to the presence of voltage-sensitive component in Xenopus sperm which may be responsible for egg-binding (Iwao and Jaffe, 1989). The ‘receptor-mediated’ theory seems to be conclusive in Xenopus because extracellular application of a sperm-mimetic ligand leads to successful egg activation.
Activation of Xenopus eggs by proteases: Possible involvement of a sperm protease in fertilization
1999, Developmental BiologyVoltage-dependent activation of frog eggs by a sperm surface disintegrin peptide
1998, Developmental Biology
- ☆
This work was supported by a grant-in-aid for Scientific Research from the Japanese Ministry of Education, Science and Culture to Y.I., by the Stuart F. Wilson Award from the University of Connecticut Health Center to L.A.J., and by NIH Grant HD 14939 to L.A.J.
- 2
Present address: Department of Physiology, University of Connecticut Health Center, Farmington, CT 06032.