Elsevier

Cellular Immunology

Volume 139, Issue 2, February 1992, Pages 292-305
Cellular Immunology

Inhibition of experimental autoimmune uveitis by retinal photoreceptor antigens coupled to spleen cells

https://doi.org/10.1016/0008-8749(92)90072-WGet rights and content

Abstract

Experimental autoimmune uveitis (EAU) and experimental autoimmune pinealitis (EAP) are CD4+ T cell-mediated inflammatory diseases of the uveal tract and retina of the eye and of the pineal gland. EAU and EAP can be induced by several retinal autoantigens including S-antigen (S-Ag) and interphotoreceptor retinoid binding protein (IRBP). In this study we investigated the effect of intravenous administration of S-Ag and IRBP coupled to syngeneic spleen cells on the development of EAU and EAP. Injection of S-Ag or IRBP coupled to spleen cells 5 days prior to immunization with native S-Ag or IRBP, respectively, was effective in preventing the induction of EAU and EAP in LEW rats. Conversely, LEW rats receiving S-Ag-coupled spleen cells and challenged with IRBP or LEW rats receiving IRBP-coupled spleen cells and challenged with S-Ag developed a severe EAU within 10 days to 2 weeks following immunization, as did all control animals receiving sham-coupled spleen cells and challenged with the two retinal antigens. The results show that the administration of retinal autoantigens coupled to spleen cells effectively protects against the development of EAU when animals are subsequently challenged with the tolerizing antigen but not when challenged with another unrelated pathogenic retinal autoantigen.

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    Supported in part by the Retina Service, Wills Eye Hospital, National Institutes of Health Grants EY5095 and BRSG5510, the Pennsylvania Lions Sight Conservation and Eye Research Foundation, Research to Prevent Blindness, Inc., the Crippled Childrens Vitreo-Retinal Research Foundation (David Meyer, M.D., Director), the Elizabeth C. King Trust, and the Grampian Health Board, Aberdeen, Scotland (H. S. Dua). H. S. Dua, M.D., Ph.D., is the Henry and Corinne Bower Retina Research Scholar. Larry A. Donoso, M.D., Ph.D., is the Thomas D. Duane Professor of Ophthalmology. Dale S. Gregerson is an RPB Senior Scientific Investigator.

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