Elsevier

Biochemical Pharmacology

Volume 34, Issue 7, 1 April 1985, Pages 1029-1033
Biochemical Pharmacology

Diurnal fluctuation and pharmacological alteration of mouse organ glutathione content

https://doi.org/10.1016/0006-2952(85)90606-9Get rights and content

Abstract

Mouse liver glutathione content showed a diurnal variation with a maximum GSH + 2 GSSG content at 6 to 10 a.m. of 62 ± 8 nmole per mg protein and a minimum of 42 ± 7 at 6 p.m. Starvation for more than 24 hr decreased the hepatic glutathione content to 22 ± 3 nmole/mg protein and abolished the diurnal rhythm. Artificial reversal of the feeding habit of the animals reversed the diurnal rhythm. Kidney, spleen and lung glutathione contents showed no such rhythm. The organ glutathione content decreased by 50% or more upon starvation. The increase of the liver glutathione content by injection of either free of liposomally entrapped GSH to starved animals was not dependent on the time of administration. The physiological maximum level could not be exceeded by this treatment. It was not possible to influence the glutathione content of kidney, lung or intestine by glutathione injections in either form. Intravenous injections of equimolar doses of 2,3-dimercaptopropanol, 2-mercaptoethanesulfonic acid, N-2-mercaptopropionylglycine, d-penicillamine, or cysteamine did not lead to any significant change in liver, kidney, spleen or lung glutathione contents 2 hr after administration. Intravenously given N-acetylcysteine, methionine, GSH or GSSG restored liver glutathione levels of starved animals to the contents observed in the fed state. The diurnal hepatic variation of GSH caused by the food intake habit of the animals may limit the capacity of the intracellular detoxication system.

References (17)

  • N.S. Kosower et al.

    Int. Rev. Cytol.

    (1978)
  • R. Hahn et al.

    Biochim. biophys. Acta

    (1978)
  • A. Wendel et al.

    Biochem. Pharmac.

    (1982)
  • A. Wendel et al.

    Biochem. Pharmac.

    (1982)
  • A. Wendel et al.

    Biochem. Pharmac.

    (1979)
  • A. Wendel et al.

    Biochem. Pharmac.

    (1981)
  • F. Tietze

    Analyt. Biochem.

    (1969)
  • R.R. Brooks et al.

    Biochem. Pharmac.

    (1981)
There are more references available in the full text version of this article.

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