The 3′-terminal nucleotide sequences of λ DNA

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Abstract

The base sequences of the 3′-termini of coliphage λ DNA have been analyzed by a new technique. Escherichia coli DNA polymerase I was used to add a single radioactive nucleotide to the 3′-OH terminus of one of the DNA strands. The DNA was then digested with pancreatic DNase I, and the resulting oligonucleotides were separated by two dimensional ionophoresis. Terminal oligonucleotides were identified by the presence of the radioactive label, and the base sequence of the labelled terminus was deduced from the base compositions of the terminal di-, tri-, tetra-, etc., oligonucleotides. It is found that the left 3′-terminus of λ DNA ends with the sequence d(pCpGpCpG) and the right 3′-terminus ends with the sequence d(pCpG).

References (18)

  • RichardsonC.C.

    J. Mol. Biol.

    (1966)
  • WeissB. et al.

    J. Mol. Biol.

    (1967)
  • WuR. et al.

    J. Mol. Biol.

    (1968)
  • WuR.

    J. Mol. Biol.

    (1970)
  • WuR. et al.

    J. Mol. Biol.

    (1971)
  • PadmanabhanR. et al.

    J. Mol. Biol.

    (1972)
  • EnglundP.T.

    J. Biol. Chem.

    (1971)
  • EnglundP.T.

    J. Mol. Biol.

    (1972)
  • SangerF. et al.

    J. Mol. Biol.

    (1965)
There are more references available in the full text version of this article.

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