The isolation and characterization of N-[9-(β-D-ribofuranosyl-purin-6-ylcarbamoyl] glycine from yeast transfer RNA☆

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Abstract

The modified nucleoside, N-[9-(β-D-ribofuranosyl)-purin-6-ylcarbamoyl] glycine, has been isolated from enzymic digests of unfractionated yeast tRNA. Structural elucidation was accomplished by detailed comparisons of natural and synthetic materials by paper chromatography, ultraviolet, nmr and mass spectra.

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☆

Part of this work was completed by M.P.S. and K.McG. in the Department of Experimental Therapeutics, Roswell Park Memorial Institute. An account of this work was given at the Pacific Slope Biochemical Conference, University of California, San Diego, June, 1970.

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