Light-dark adaptation of bacteriorhodopsin in Triton-treated purple membrane
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Cited by (62)
Existence of two substates in the O intermediate of the bacteriorhodopsin photocycle
2022, Biochimica et Biophysica Acta - BiomembranesCitation Excerpt :A peculiar feature of the photoreaction of wild-type bR is that the light-initiated interconversion from the trans to the 13-cis isomer is strictly inhibited in an aqueous suspension of purple membrane. However, this inhibition becomes loose when bR is solubilized or when some residues in the retinal-binding pocket are mutated [7,8]. The cause of this loosening has remained to be elucidated.
Tuning the Photocycle Kinetics of Bacteriorhodopsin in Lipid Nanodiscs
2015, Biophysical JournalCitation Excerpt :Alternatively, one could also attribute the spectral shifts of nbR to the different population of the 13-cis and all-trans retinal in the light-adapted form. An early study by Casadio et al. (46) found different ratios of 13-cis/all-trans retinal in light-adapted form in PM and Triton X-100 micelles. The percentage of the all-trans retinal in PM (82%) is higher than that in Triton X-100 solubilized bR (62%) (46).
Primary conformation change in bacteriorhodopsin on photoexcitation
2009, Biophysical JournalCitation Excerpt :The lifetime of the excited state is known to be different at pH >9, as reported by Logunov et al. (32). At pH 7, light-adapted bR mainly has an all-trans structure as reported in previous works (33,34). In Fig. 6 of Casadio et al. (33), absorption spectra of 13-cis and all-trans are peaked at 539 nm and 562 nm, respectively.
Photochromicity of Anabaena sensory rhodopsin, an atypical microbial receptor with a cis-retinal light-adapted form
2005, Journal of Biological ChemistrySpectroscopic techniques in the study of membrane solubilization, reconstitution and permeabilization by detergents
2000, Biochimica et Biophysica Acta - BiomembranesCitation Excerpt :It may take hours or even days to be completed, when the corresponding process takes place within seconds in most other membranes. The slow kinetics was noted by Dencher and Heyn [35] and by Casadio et al. [36], and studied in more detail by Viguera et al. [23]. It should be noted that the ‘purple membrane’ consists actually of patches of a two-dimensional crystalline array of bacteriorhodopsin and lipids interspersed in the bacterium plasma membrane.
A novel three-dimensional crystal of bacteriorhodopsin obtained by successive fusion of the vesicular assemblies
1998, Journal of Molecular Biology