A simple enzymatic method for the preparation of radiolabeled erucoyl-CoA and other long-chain fatty acyl-CoAs and their characterization by mass spectrometry☆
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Cited by (102)
Acyl-CoA:diacylglycerol acyltransferase: Properties, physiological roles, metabolic engineering and intentional control
2022, Progress in Lipid ResearchKinetics of eicosapentaenoic acid in brain, heart and liver of conscious rats fed a high n-3 PUFA containing diet
2013, Prostaglandins Leukotrienes and Essential Fatty AcidsThe multigene family of lysophosphatidate acyltransferase (LPAT)-related enzymes in Ricinus communis. Cloning and molecular characterization of two LPAT genes that are expressed in castor seeds
2013, Plant ScienceCitation Excerpt :Non-radioactive acyl-CoAs and sn-1-oleoyl-sn-glycerol 3-phosphate (18:1-LPA) were obtained from Sigma–Aldrich Corp. Radioactively labelled [14C]sn-1-oleoyl-sn-glycerol 3-phosphate (55 mCi/mmol) was purchased from American Radioactive Corp. 18:1-OH-CoA was synthesized following the enzymatic method by Taylor et al. [45] using the acyl-CoA synthetase from Pseudomonas sp. (Sigma–Aldrich). Analysis and quantification of the purified acyl-CoA product were performed by HPLC, using 18:1-CoA as standard.
Fifteen weeks of dietary n-3 polyunsaturated fatty acid deprivation increase turnover of n-6 docosapentaenoic acid in rat-brain phospholipids
2012, Biochimica et Biophysica Acta - Molecular and Cell Biology of LipidsCitation Excerpt :Peaks were identified from the retention times of acyl-CoA standards. The acyl-CoA standard for DPAn-6 was prepared from the free fatty acid and free CoA by an enzymatic method [45]. Endogenous acyl-CoA concentrations (nmol/g brain) were calculated by direct proportional comparison with the peak area of the 17:0-CoA internal standard.
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NRCC No. 30710.