Abstract
In this communication luminescent bioconjugated human serum albumin nanostructures (HSA NPs) with tiny ultraluminescent gold core–shell silica nanoparticles (Au@SiO2-Fl) were designed with enhanced bi-coloured luminescence properties. The HSA NPs were obtained from Human Serum Albumin free (HSA free) through the desolvation method, and Au@SiO2-Fl, through modified Turkevich and Störber methods. In this manner, porous HSA Nanostructures of 150.0–200 nm and Au@SiO2-Fl 45.0 nm final diameters were obtained. Both methodologies and structures were conjugated to obtain modified Nanocomposites based on tiny gold cores of 15 nm surrounded with well spatial Nanostructured architectures of HSA (d15 Au@SiO2-Fl-HSA) that generated variable nanopatterns depending on the modified methodology of synthesis applied within colloidal dispersions. Therefore, three methodologies of non-covalent conjugation were developed. In optimal conditions, through Transmission Electronic Microscopy (TEM), well resolved multilayered nano-architectures with a size 190.0–200 nm in average with variable contrast depending of the focused nanomaterial within the nanocomposite were shown. Optimized nanoarchitecture was based on a template tiny gold core–shell surrounded by nanostructured HSA NPs (d15 Au@SiO2-Fl-HSA). In this manner, the NanoImaging generated by laser fluorescence microscopy permitted to record improved optical properties and functionalities, such as: (i) enhanced ultraluminescent d15 Au@SiO2-Fl-HSA composites in comparison to individual components based on Metal Enhanced Fluorescence (MEF); (ii) diminished photobleaching; (iii) higher dispersibility; (iv) higher resolution of single bright nano-emitters of 210.0 nm sizes; and (v) enhanced bi-coloured Bio-MEF coupling with potential non-classical light delivery towards other non-optical active biostructures for varied applications. The characterization of these nanocomposites allowed the comparison, evaluation and discussion focused on new properties generated and functionalities based on the incorporation of different types of tuneable materials. In this context, the biocompatibility, Cargo confined spaces, protein-based materials, optical transparent could be highlighted, as well as optical active materials. Thus, the potential applications of nanotechnology to both nanomedicine and nano-pharmaceutics were discussed.
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Acknowledgements
Special thanks to the Secretary of Science and Technology (SeCyT) of the National University of Cordoba (UNC), Argentina, for the research grant provided. As well especially thanks to Professor Denis Boudreau from COPL at Laval University, Québec, Canada, for the long standing Research Collaboration in progress; as well as to all the Canadian grants that permit it. In addition, it is greatly acknowledged the visit to Professor Jesse Greener Laboratory, in the Département de Chimie forming part of the CQMF (Quebéc Center for Functional Materials) and CERMA (Center for Research on Advanced Materials), at Université Laval, Québec, Canada. Similarly, special thanks are also given to professor Valeria Amé from Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas (Center of Clin. Biochem. and Immunology Research, Dep. of Clinical Biochem., Faculty of Chem. Sci.) UNC, Argentina. Moreover, especial thanks to Professor Daniela Quinteros from Unidad de Investigación y Desarrollo en Tecnología Farmacéutica (UNITEFA) (Unit of Research and Development in Pharmaceutical Technology), at Departamento de Ciencias Farmacéuticas, Facultad de Ciencias Químicas (Dep. of Pharmaceutical Sciences, Faculty of Chemical Sci.), from UNC, and her Research Group. Finally, It is acknowledged to all the related supporters of the recent Entrepreneurship-Start-Up:“Bio-highlighting solutions” leaded in progress by A. G. B. et al., since “Awarded Prix Ideas Challenge 2010”, Entrepreneuriat ULaval–Université Laval, Laval University, Quebec, Canada. (https://www.eul.ulaval.ca/). In similar manner to all collaborators in the Research Group-In-Progress focused on “Design and synthesis of new Optical active Nanostructures with Ultraluminescent applications applied for Photonics materials, Biophotonics, Nano-, Microfluidic systems, and Devices”; in Collaboration with COPL, Ulaval, Canada, and other International partners.
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Palacios, L.R.G., Martinez, S.M., Tettamanti, C.S. et al. Bi-coloured enhanced luminescence imaging by targeted switch on/off laser MEF coupling for synthetic biosensing of nanostructured human serum albumin. Photochem Photobiol Sci 22, 2735–2758 (2023). https://doi.org/10.1007/s43630-023-00483-5
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DOI: https://doi.org/10.1007/s43630-023-00483-5