Abstract
Momordica charantia L., Cucurbitaceae, known mainly as karela, bitter gourd or bitter melon, and balsam pear, is used for antihyperglycemic, antibacterial, antiviral, antitumor, immunomodulation, antioxidant, antidiabetic, anthelmintic, antimutagenic, antiulcer, antilipolytic, antifertility, hepatoprotective, anticancer, and anti-inflammatory and wound healing. This study aimed to elucidate the differences in the metabolites of 70% methanol extracts of M. charantia seeds and fruit using untargeted metabolomics. Liquid chromatography coupled to quadrupole time-of-flight mass spectrometry–based analysis of the extracts for both seed and fruit was performed using a C-18 column. Differences were observed in seed and fruit extracts, which were visualized using principal component analysis plots. (R)-Salsolinol, pantetheine, coumarin, tryptamine, lysophospholipidPC(O-18:0), glucosylceramide, pyroglutamic acid, and presqualene diphosphate in the seed and fruit of M. charantia were detected in different levels. The amount of lysophospholipidPC(O-18:0) (lysoPC(O-18:0)) and glucosylceramide is high in the fruit, while the amount of (R)-salsolinol, pantetheine, coumarin, tryptamine, presqualene diphosphate, and pyroglutamic acid is high in the seed. These primary untargeted metabolomic results revealed that the different pharmacological effects may be related to the variable amounts of some specific metabolites in seeds and fruits.
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Ahmad Z, Zamhuri KF, Yaacob A, Siong CH, Selvarajah M, Ismail A, Hakim MN (2012) In vitro anti-diabetic activities and chemical analysis of polypeptide-k and oil isolated from seeds of Momordica charantia (bitter gourd). Molecules 17:9631–9640. https://doi.org/10.3390/molecules17089631
Aremu M, Waziri A, Faleye F, Magomya A, Okpaegbe U (2019) Lipids profile of bitter melon (Momordica charantia L.) fruit and ebony (Diospyros mespiliformis Hochst ex A. DC.) tree fruit pulp. Bangladesh J Multidiscip Sci Res 54:367–374. https://doi.org/10.3329/bjsir.v54i4.44571
Daniel P, Supe U, Roymon M (2014) A review on phytochemical analysis of Momordica charantia. Int J Adv Pharm Biol Chem 3:214–220
Du J, Chan LY, Poth AG, Craik DJ (2019) Discovery and characterization of cyclic and acyclic trypsin inhibitors from Momordica dioica. J Nat Prod 82:293–300. https://doi.org/10.1021/acs.jnatprod.8b00716
Dunn WB, Bailey NJ, Johnson HE (2005) Measuring the metabolome: current analytical technologies. Analyst 130:606–625. https://doi.org/10.1039/B418288J
Eto M, Watanabe K, Chonan N, Ishii K (1987) Lowering effect of pantethine on plasma beta thromboglobulin and lipids in diabetes mellitus. Artery 15:1–12
Hall RD, Brouwer ID, Fitzgerald MA (2008) Plant metabolomics and its potential application for human nutrition. Physiol Plant 132:162–175. https://doi.org/10.1111/j.1399-3054.2007.00989.x
Hegeman AD (2010) Plant metabolomics—meeting the analytical challenges of comprehensive metabolite analysis. Brief Funct Genomics 9:139–148. https://doi.org/10.1093/bfgp/elp053
Hong J, Yang L, Zhang D, Shi J (2016) Plant metabolomics: an indispensable system biology tool for plant science. Int J Mol 17:767. https://doi.org/10.3390/ijms17060767
Inubushi T, Kamemura N, Oda M, Sakurai J, Nakaya Y, Harada N, Suenaga M, Matsunaga Y, Ishıdoh K, Katunuma N (2012) L-tryptophan suppresses rise in blood glucose and preserves insulin secretion in type-2 diabetes mellitus rats. J Nutr Sci 58:415–422. https://doi.org/10.3177/jnsv.58.415
Islam J, Shirakawa H, Nguyen TK, Aso H, Komai M (2016) Simultaneous analysis of serotonin, tryptophan and tryptamine levels in common fresh fruits and vegetables in Japan using fluorescence HPLC. Food Biosci 13:56–59. https://doi.org/10.1016/j.fbio.2015.12.006
Jia S, Shen M, Zhang F, Xie J (2017) Recent advances in Momordica charantia: functional components and biological activities. Int J Mol 18:2555. https://doi.org/10.3390/ijms18122555
Kahraman E, Kaykın M, Şahin Bektay H, Güngör S (2019) Recent advances on topical application of ceramides to restore barrier function of skin. Cosmetics 6:52. https://doi.org/10.3390/cosmetics6030052
Kumar D, Kumar S, Shekhar C (2020) Role of bitter gourd (Momordica charantia L.) in human health strengthening and regulate different diseases. J Pharmacogn Phytochem 9:895–899
Li H, Yao Y, Li L (2017) Coumarins as potential antidiabetic agents. J Pharm Pharmacol 69:1253–1264. https://doi.org/10.1111/jphp.12774
Melzig MF, Putscher I, Haber H, Rottmann M, Zipper J (1998) Toxicity and pharmacological effects of salsolinol in different cultivated cells. In: Moser A (ed) Pharmacology of endogenous neurotoxins: a handbook. Birkhäuser, Boston, pp 253–266
Moco S, Vervoort J, Bino RJ, De Vos RC, Bino R (2007) Metabolomics technologies and metabolite identification. Trends Analyt Chem 26:855–866. https://doi.org/10.1016/j.trac.2007.08.003
Oksman-Caldentey K-M, Inzé D, Orešič M (2004) Connecting genes to metabolites by a systems biology approach. Proc Natl Acad Sci 101:9949–9950. https://doi.org/10.1073/pnas.0403636101
Ooi CP, Yassin Z, Hamid TA (2012) Momordica charantia for type 2 diabetes mellitus. Cochrane Database Syst Rev 15:CD007845. https://doi.org/10.1002/14651858.CD007845.pub3
Shu C-H, Jaiswal R, Peng Y-Y, Liu T-H (2022) Improving bioactivities of Momordica charantia broth through fermentation using mixed cultures of Lactobacillus plantarum, Gluconacetobacter sp. and Saccharomyces cerevisiae. Process Biochem 117:142–152. https://doi.org/10.1016/j.procbio.2022.03.023
Su M-J, Tsai C-Y, Ruan C-T, Yeh C-H, Hsu C-M, Task S-F, Lee SS (2018) Studies of the hypoglycemic action of salsolinol in type 2 diabetic mice. Proc Annual Meeting of the Japanese Pharmacological Society WCP2018 (The 18th World Congress of Basic and Clinical Pharmacology). July 1 – 6, 2018, Kyoto, Japan. https://doi.org/10.1254/jpssuppl.WCP2018.0_OR28-1
Tang J-W, Xiong X-S, Qian C-L, Liu Q-H, Wen P-B, Shi X-Y, Dereje SB, Zhang X, Wang L (2021) Network pharmacological analysis of ethanol extract of Morus alba linne in the treatment of type 2 diabetes mellitus. Arab J Chem 14:103384. https://doi.org/10.1016/j.arabjc.2021.103384
Thangavel P, Ramachandran B, Chakraborty S, Kannan R, Lonchin S, Muthuvijayan V (2017) Accelerated healing of diabetic wounds treated with L-glutamic acid loaded hydrogels through enhanced collagen deposition and angiogenesis: an in vivo study. Sci Rep 7:10701. https://doi.org/10.1038/s41598-017-10882-1
Yang P, Li X, Shipp MJ, Shockey JM, Cahoon EB (2010) Mining the bitter melon (Momordica charantia L.) seed transcriptome by 454 analysis of non-normalized and normalized cDNA populations for conjugated fatty acid metabolism-related genes. BMC Plant Biol 10:250. https://doi.org/10.1186/1471-2229-10-250
Yang X, Chen F, Huang G (2020) Extraction and analysis of polysaccharide from Momordica charantia. Ind Crops Prod 153:112588. https://doi.org/10.1016/j.indcrop.2020.112588
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MÇ, AAB: conceptualization; MÇ, AAB, DE: methodology; DE: sample collection, extraction, analysis, data analysis; BBF: data analysis; MÇ, DE, AAB: writing — original draft; MÇ, AAB: supervision. This study was a part of DE M.Sc. thesis. All authors have read the final manuscript and approved the submission.
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Eneş, D., Fidan, B.B., Başaran, A.A. et al. Comparative Plant Metabolomics of Momordica charantia Seeds and Fruits. Rev. Bras. Farmacogn. (2024). https://doi.org/10.1007/s43450-024-00539-2
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DOI: https://doi.org/10.1007/s43450-024-00539-2