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Construction and optimization of boldenone synthesis from androstenedione catalyzed by a dual-enzyme system

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Abstract

Boldenone is a protein-assimilating androgen steroid that can promote protein synthesis, support nitrogen storage, and enhance renal erythropoietin release. The industrial production of boldenone mainly relies on chemical synthesis, which has various problems, such as a complex conversion process, excessive byproducts, and serious environmental pollution. Therefore, it is of great significance to explore a new biosynthetic route. Recently, the enzymatic synthesis of steroid compounds has been performed more frequently than in the past. In this work, boldenone was produced from androstenedione (AD) in two steps by a dual-enzyme cascade of 17β-hydroxysteroid dehydrogenase (17β-HSD) and 3-sterone-Δ1-dehydrogenase (KstD). The conversion efficiency of three isoenzymes of 17β-HSD from Mycobacterium sp. LY-1 for substrate AD was first analyzed. After that, the 17β-HSD2 with high selectivity and specificity for AD was screened and co-expressed with KstD3 in Escherichia coli BL21 to construct a dual-enzyme catalytic system. The results showed that the synthesis of boldenone from AD could be achieved by constructing the dual-enzyme expression system of 17β-HSD and KstD, as we determined that the concentration of boldenone reached 24.3 mg/L. To further improve the synthesis efficiency of boldenone, the expression conditions of the dual-enzyme system were optimized, and the concentration of boldenone reached 31.9 mg/L. The exploration of this route will provide a foundation for the efficient enzymatic synthesis of boldenone.

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The datasets used or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

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Funding

This project was supported by National Key Research and Development Program of China (No. 2019YFA0905300); the National Natural Science Foundation of China (No. 22078126); Qing Lan Project in Jiangsu Province; Fundamental Research Funds for Central Universities of China (No. JUSRP221025).

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Authors and Affiliations

  1. School of Life Sciences and Health Engineering, Jiangnan University, Wuxi, 214122, Jiangsu, China

    Y. Liang, H. Li, L. Y. Xu, J. X. Zhang & J. S. Shi

  2. Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, Jiangsu, China

    W. Liu & Z. H. Xu

  3. Tianjin Pharmaceutical Research Institute Limited Company, Tianjin, 300301, China

    L. Y. Chen & S. L. Wang

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Contributions

Liang Y carried out the studies, participated in the sequence alignment and drafted the manuscript. Zhang JX, Xu LY participated in the sequence alignment. Liu W participated in the design of the study and performed the statistical analysis. Li H, Shi JS, and Xu ZH conceived of the study, and participated in its design and coordination and helped to draft the manuscript. Chen LY and Wang SL provided some of the experimental material. All authors read and approved the final manuscript.

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Correspondence to H. Li or J. S. Shi.

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Liang, Y., Li, H., Liu, W. et al. Construction and optimization of boldenone synthesis from androstenedione catalyzed by a dual-enzyme system. Syst Microbiol and Biomanuf 4, 783–793 (2024). https://doi.org/10.1007/s43393-023-00187-y

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  • DOI: https://doi.org/10.1007/s43393-023-00187-y

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