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Melatonin Attenuates Peroxynitrite-Induced Meiosis Dysfunction in Porcine Oocytes

  • Reproductive Biology: Original Article
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Abstract

A high level of reactive oxygen species (ROS) is widely considered one of the major causes of oocyte quality decline. Peroxynitrite is known as a powerful oxidant, which could induce multiple physical diseases. Recently, emerging pieces of evidences indicate that melatonin effectively promotes the development of oocytes, although the specific work mechanism remains to be further clarified. In this study, it was shown that peroxynitrite increased the level of ROS in porcine oocytes, which induced the apoptosis of oocytes, thereby leading to the obstruction of spindle assembly, depolymerization of actin, and decrease of polar body expulsion. These negative effects contributed to the failure of meiosis and ultimately blocked the maturation of porcine oocytes. As expected, it was found that melatonin effectively removed the accumulated ROS in oocytes, preventing oocytes from peroxynitrite-induced oocyte maturation failure, which might provide a novel approach to improve female livestock reproduction and cure female infertility in clinical practice.

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Funding

This study was supported by the National Natural Science Foundation of China (grant no. 31970413), National Key R&D Program of China (grant no. 2018YFC1200201), and start-up grant from Nanjing Agricultural University (grant no. 804090).

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Authors and Affiliations

  1. Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China

    Yan Cao, Rongyang Li, Weijian Li, Honglin Liu & Yafei Cai

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  1. Yan Cao
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  2. Rongyang Li
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  3. Weijian Li
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  4. Honglin Liu
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  5. Yafei Cai
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Contributions

All authors were involved in designing the experiments. Yan Cao performed the experiments, analyzed the data, and drafted the manuscript. Rongyang Li and Weijian Li contributed to the sample collection. Honglin Liu contributed the reagents. Yafei Cai revised the manuscript.

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Correspondence to Yafei Cai.

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The authors declare that they have no conflict of interest.

Ethical Approval

All procedures with porcine ovaries were conducted in accordance with the guidelines of the Animal Research Institute Committee at Nanjing Agricultural University (SYXK-2017-0027).

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Cao, Y., Li, R., Li, W. et al. Melatonin Attenuates Peroxynitrite-Induced Meiosis Dysfunction in Porcine Oocytes. Reprod. Sci. 28, 1281–1289 (2021). https://doi.org/10.1007/s43032-020-00331-2

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  • DOI: https://doi.org/10.1007/s43032-020-00331-2

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