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The Expression and Contribution of SRCs with Preeclampsia Placenta

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Abstract

The steroid hormones act by binding to their receptors and subsequently interacting with coactivators. Several classes of coactivators have been identified and shown to be essential in estradiol (E2) responsiveness. The major coregulators are the p160 steroid receptor coactivator (SRC) family. Although the function of SRCs in other organs has been well studied, it has not been thoroughly studied in the placenta. In addition, the correlation between preeclampsia (PE) and SRCs has not been examined previously. Therefore, we compared the expression patterns of SRCs in normal and PE placentas. In human PE placental tissues, SRC-1 mRNA, and protein levels were downregulated in the PE group. In addition, to assess the expression of SRCs in a PE environment, we used Reduced Uterine Perfusion Pressure (RUPP) model and placental cells were cultured in hypoxia condition. SRC-1 proteins were reduced in the placenta of PE-like rat RUPP model. Furthermore, SRCs proteins were significantly downregulated in hypoxia-grown placental cells. To examine the interaction between estrogen receptors (ERs) and SRC-1 protein, we performed co-immunoprecipitation. The interaction of SRC-1 with ERα was significantly stronger than that with ERβ. In PE placenta, the interaction of both ERα and ERβ with SRC-1 was stronger than that in normal placenta. In summary, our results demonstrate that expression levels of SRC-1, not SRC-2 and SRC-3, were decreased in hypoxia-induced PE placenta, which may further reduce the signaling of sex steroid hormones such as E2. The dysregulated signaling of E2 by SRC-1 expression could be associated with the PE placental symptoms of patients.

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Acknowledgments

This study was supported by Research institute for Convergence of biomedical science and technology (30-2016-014), Pusan National University Yangsan Hospital. The biospecimens and data used for this study were provided by the Biobank of Pusan National University Hospital, a member of the Korea Biobank Network.

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Author notes

  1. Jea Sic Jeong and Dong Hyung Lee contributed equally to this work.

Authors and Affiliations

  1. Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, 50 Cheonghak-ri, Samrangjin-eup, Miryang, Gyeongsangnam-do, 627-706, South Korea

    Jea Sic Jeong, Jae-Eon Lee, Sung-Min An, Dae Youn Hwang, Seung Yun Yang & Beum-soo An

  2. Department of Obstetrics and Gynecology, Research Institute for Convergence of Biomedical Science and Technology, Pusan National University College of Medicine, Yangsan, Gyeongnam, 50612, South Korea

    Dong Hyung Lee & Seung Chul Kim

  3. Department of Bioenvironmental Energy, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Busan, South Korea

    Pyong In Yi

  4. College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon, South Korea

    Geun-Shik Lee

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  1. Jea Sic Jeong
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Correspondence to Seung Chul Kim or Beum-soo An.

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Jeong, J.S., Lee, D.H., Lee, JE. et al. The Expression and Contribution of SRCs with Preeclampsia Placenta. Reprod. Sci. 27, 1513–1521 (2020). https://doi.org/10.1007/s43032-020-00142-5

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  • DOI: https://doi.org/10.1007/s43032-020-00142-5

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