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Delivery methods for CRISPR/Cas9 gene editing in crustaceans

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Abstract

In this mini-review, we provide an up-to-date overview of the delivery methods that have been used for CRISPR/Cas9 genomic editing in crustacean species. With embryonic microinjection as the main workforce for delivering CRISPR/Cas9 reagents, biologists working with crustacean species have to tackle the technical challenges involved in microinjection. We use examples of three crustacean species (the branchiopod Daphnia, amphipod Parhyale hawaiensis, and decapod Exopalaemon carinicauda) to provide a technical guide for embryonic microinjection. Moreover, we outline two potentially useful new techniques for delivering CRISPR/Cas9 components into crustaceans, i.e., Receptor-Mediated Ovary Transduction of Cargo (ReMOT Control) and electroporation.

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Acknowledgements

This work is supported by NIH Grant R35GM133730 to Sen Xu.

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Authors and Affiliations

  1. Department of Biology, University of Texas at Arlington, 501 S. Nedderman Dr, Arlington, TX, 76019, USA

    Sen Xu, Thinh Phu Pham & Swatantra Neupane

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  1. Sen Xu
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  2. Thinh Phu Pham
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  3. Swatantra Neupane
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SX, TP and SN reviewed literature and wrote the manuscript. The manuscript has been approved by all authors.

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Correspondence to Sen Xu.

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The authors declare no conflicts of interests.

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No animal and human rights are involved in this article.

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Edited by Jiamei Li.

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Xu, S., Pham, T.P. & Neupane, S. Delivery methods for CRISPR/Cas9 gene editing in crustaceans. Mar Life Sci Technol 2, 1–5 (2020). https://doi.org/10.1007/s42995-019-00011-4

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