Abstract
Soil amino sugars have been widely used to evaluate the potential roles of microbes in mediating soil carbon (C) cycling and various pretreatment methods were used for its extraction. However, few studies assessed their potential influences on the soil amino sugar extraction. In this study, we investigated the effects of sample storage method and grinding on amino sugar extraction across different climatic zones and land uses. Results showed that the concentrations of soil amino sugars varied greatly among sample pretreatments and their impacts were highly dependent on climatic condition and land use. Specifically, higher concentrations of amino sugars were extracted from field-moist samples than dried samples in subtropical grassland, temperate forest and arable land with no significant differences among storage methods for the samples from subtropical forest, arable land, and temperate grassland. Moreover, grinding improved the extraction efficiency of amino sugars for the dried soils. Due to the reduced extraction concentration in dried soils, field-moist samples were recommended in priority. For the dried soils used for the long-term storage, grinding can be an option to improve the extraction efficiency. Such information will be valuable for reducing the uncertainty and improving the accuracy during the determination of soil amino sugars.
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Acknowledgments
We thank Dr. Lujun Li, Dr. Chao Wang and Mr. Dongbo Wang for their assistance in sample collection. This work was supported by the National Natural Science Foundation of China (Grant Nos. 41771278, 41571249 and U1701246), Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Grant No. GML2019- ZD0408), Science and Technology Program of Guangzhou City (Grant No. 201707010344), Open Foundation of the State Key Laboratory of Loess and Quaternary Geology (Grant No. SKLLQG1917) and Youth Innovation Promotion Association of Chinese Academy of Sciences.
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Mou, Z., Kuang, L., Yan, B. et al. Influences of sample storage and grinding on the extraction of soil amino sugars. Soil Ecol. Lett. 2, 157–163 (2020). https://doi.org/10.1007/s42832-020-0031-9
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DOI: https://doi.org/10.1007/s42832-020-0031-9