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Diagnostic performance of the bioline dengue duo rapid test on symptomatic patients assisted at Armed Forces Hospital (Hfa) in Brasília, Brazil

  • Medicine and Public Health - Research Paper
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Brazilian Journal of Microbiology Aims and scope Submit manuscript

Abstract

Dengue necessitates accurate diagnosis. Rapid tests such as Bioline™ DENGUE DUO have gained traction, but validation in specific populations is essential. This study aimed to evaluate the performance of the Bioline™ test, alongside assessing the socio-epidemiological profile of symptomatic patients in a Brasília Military Hospital. The serum of 404 symptomatic patients was analyzed by the Bioline™ DENGUE DUO test, followed by Dengue virus detection and discrimination of the four serotypes by RT-qPCR. Accuracy was assessed using parameters including sensitivity (S), specificity (E), positive and negative predictive values (PPV and NPV), and positive (RV +) and negative (RV-) likelihood ratios. The NS1 component exhibited a sensitivity of 70.37%, a specificity of 97.30%, and an overall efficiency of 90.10% when compared to RT-qPCR as the gold standard. The IgM component demonstrated a sensitivity of 26.85%, a specificity of 89.53%, and an overall efficiency of 72.77% when compared to RT-qPCR as the gold standard. The IgG component demonstrated a sensitivity of 23.15%, a specificity of 68.92%, and an overall efficiency of 56.68% when compared to RT-qPCR as the gold standard. Several rapid tests are commercially available. However, considering variations across regions and demographic groups, it is important to question their accuracy in specific populations. Rapid tests are important screening tools, but they can have limitations for the certainty of diagnosis. Bioline™ DENGUE DUO displayed good specificity, but sensitivity was slightly below optimal levels. While helpful for confirming dengue, improvements are needed to effectively rule out the disease.

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Data availability

The data supporting the results of this study will be made available by the corresponding author upon reasonable request.

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Funding

This work was funded by the Program of Academic Cooperation in National Defense (PROCAD), Coordination for the Improvement of Higher Education Personnel (CAPES), and National Research Council (CNPq).

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Authors and Affiliations

  1. Department of Genetics, Federal University of Goias, Avenida Esperança, s/n, Câmpus Samambaia, Goiania, GO, 74.690-900, Brazil

    Leani Falcão Gomes, Lívia do Carmo Silva, Juliana Santana de Curcio, Adriano Roberto Vieira de Sousa, Juliana Ramos do Carmo & Elisângela de Paula Silveira-Lacerda

  2. Research Division of Technical Teaching and Research Directorate, Armed Forces Hospital, Brasilia, DF, Brazil

    Leani Falcão Gomes & Júlia Viegas Mundim

  3. Department of Tropical Medicine and Dermatology, Federal University of Goias, Goiania, GO, Brazil

    Marco Tulio Garcia-Zapata

  4. Department of Biochemistry and Molecular Biology, Federal University of Goias, Goiania, GO, Brazil

    Carlos Eduardo Anunciação

Authors
  1. Leani Falcão Gomes
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  2. Júlia Viegas Mundim
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  3. Lívia do Carmo Silva
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  4. Juliana Santana de Curcio
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  5. Adriano Roberto Vieira de Sousa
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  6. Marco Tulio Garcia-Zapata
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  7. Carlos Eduardo Anunciação
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  8. Juliana Ramos do Carmo
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  9. Elisângela de Paula Silveira-Lacerda
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Contributions

All authors contributed to the study conception and design. Material preparation, sample assembly, data collection, analysis and the first version of the manuscript were carried out by Leani F. Gomes. Review, formatting and textual standardization were carried out by Júlia. V Mundim, Lívia do C Silva, Juliana de Curcio, Adriano Roberto V. de Sousa, Marco Tulio Zapata, Carlos Eduardo Anunciação, Juliana R. do Carmo and Elisângela de P. Silveira-Lacerda. All authors commented on previous versions of the manuscript and approved the final manuscript.

Corresponding author

Correspondence to Elisângela de Paula Silveira-Lacerda.

Ethics declarations

Ethical approval

In accordance with CNS Resolution 466/2012, precautions were taken to preserve the anonymity of the personal data collected. This study is related to the research project “Implementation of a sentinel laboratory for arbovirus surveillance at Armed Forces Hospital” submitted and approved by the Research Ethics Committee of the Federal University of Goias (No. 4.342.314) and to the HFA (No. 4.618.807).

Informed consent

Informed consent was obtained from all study participants by the HFA Research laboratory, and samples were packaged and processed anonymously with internal codes as agreed.

Competing interest

The author reports no financial interests or other conflicts of interest in this work.

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Responsible Editor: Mauricio Nogueira

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Gomes, L.F., Mundim, J.V., do Carmo Silva, L. et al. Diagnostic performance of the bioline dengue duo rapid test on symptomatic patients assisted at Armed Forces Hospital (Hfa) in Brasília, Brazil. Braz J Microbiol (2024). https://doi.org/10.1007/s42770-024-01351-9

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