Abstract
Objective
To evaluate the antimicrobial activity of Brazilian honeys against oral microorganisms. Design: Organic honeys (OH-1 to OH-8) were diluted (%-w/v) and sterilized by filtration. Antimicrobial activity was defined by determining MIC and CBM against oral Streptococcus. The component responsible for the antimicrobial action was defined by a catalase assay. Antibiofilm activity was evaluated against the monospecies biofilm of Streptococcus mutans (ATCC 700610).
Results
OHs showed antimicrobial activity principally OH-1, OH-2, OH-3, and OH-7 with MIC values ranging between 10 and 25%. The mechanism of action occurs mainly by hydrogen peroxide produced by honey enzymes. OH-1, OH-2, and OH-7 showed total biofilm destruction at low concentrations.
Conclusion
Brazilian honeys have promising antimicrobial and antibiofilm activity with the potential to control oral microbiota.
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Acknowledgements
The authors are thankful to Breyer e Cia Ltda. — Produtos das Abelhas for providing the organic honey samples used in this study.
Funding
This work was supported by the [Coordenação de Aperfeiçoamento de Pessoal de Nível Superior 1]; under Grant [number: 88887476194/2020-00]; [Conselho Nacional de Desenvolvimento Científico e Tecnológico 2] under Grant [141129/2017-4] and under Grant [306673/2019-3].
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Conceptualization, Diego Romário da Silva and Pedro Luiz Rosalen; Methodology, Diego Romário da Silva, Janaína de Cássia Orlandi Sardi and Marcelo Franchin; Investigation, Diego Romário da Silva and Janaína de Cássia Orlandi Sardi; Resources, Pedro Luiz Rosalen and Severino Matias Alencar; Data Curation, Diego Romário da Silva; Writing – Original Draft Preparation, Diego Romário da Silva, Andréa Cristina Barbosa da Silva and Fabiane Cruz Vieira; Writing – Review & Editing, Diego Romário da Silva and Pedro Luiz Rosalen.; Supervision, Pedro Luiz Rosalen; Project Administration, Pedro Luiz Rosalen; Funding Acquisition, Pedro Luiz Rosalen.
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Romário-Silva, D., Franchin, M., Alencar, S.M. et al. Antimicrobial and antibiofilm activities of Brazilian organic honey against oral microorganisms. Braz J Microbiol (2024). https://doi.org/10.1007/s42770-024-01343-9
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DOI: https://doi.org/10.1007/s42770-024-01343-9