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Extraction, characterization, and biological activities of exopolysaccharides from plant root soil fungus Fusarium merismoides A6

  • Environmental Microbiology - Research Paper
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Abstract

The exploration of polysaccharides from microorganisms is of great importance. In this study, a new type of exopolysaccharide excreted by Fusarium merismoides A6 (FM-EPS) was isolated, and the extraction conditions were optimized using a response surface methodology (RSM). The extraction temperature at 0 °C, a precipitation time of 7.83 h, and an ethanol precipitation concentration of 77.64% were predicted and proved to be the best extraction conditions with the maximum extraction yield of 0.74 g/mL. Then, two fractions of F. merismoides A6 exopolysaccharides (FM-EPS1 and FM-EPS2) were obtained through DEAE Sepharose fast flow column chromatography. As indicated by monosaccharide composition analysis, both fractions mainly consisted of mannose, glucose, galactose, and ribose, with an average molecular weight of 5.14 × 104 and 6.50 × 104 g/mol, respectively. FT-IR and NMR spectroscopy indicated the FM-EPSs had both α- and β-glycosidic bonds. Moreover, the determination of antioxidant and antiproliferative activities in vitro proved that FM-EPSs had good antioxidant activities and antiproliferation activities. FM-EPS1 showed stronger antioxidant activities than FM-EPS2. FM-EPS2 showed antiproliferation activities on HeLa and HepG2 cells, while FM-EPS1 had no obvious antiproliferative activity. Therefore, FM-EPSs could be explored as potential antioxidant and anticancer agent applied in food, feed, nutraceutical, pharmaceutical, cosmetics, and chemical industries.

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Funding

We greatly appreciate the financial support from Shandong Provincial Science Fund for Distinguished Young Scholars (No. ZR2020JQ11), Young Taishan Scholars (grant number TSQN201909159), and the Natural Science Foundation of Shandong Province (No. ZR2019BC060).

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Author notes

  1. Guoqiang Chen and Zhenxiang Xu contributed equally to this work.

Authors and Affiliations

  1. CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China

    Guoqiang Chen, Zhenxiang Xu, Fan Wang, Lijuan Liu, Xiao Men & Haibo Zhang

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Guoqiang Chen & Fan Wang

  3. Key Laboratory of Shandong Microbial Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, China

    Zhenxiang Xu, Jinglong Li & Kai Zheng

  4. College of Life Science, Qingdao University, Qingdao, 266071, China

    Yuxi Wei

  5. Great Wall Cigar Factory Technology Development Center of China Tobacco Sichuan, Shifang, 618400, China

    Liang Zhang

  6. Yunnan Academy of Tobacco Sciences, Kunming, 650106, China

    Lijun Wu

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  1. Guoqiang Chen
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Chen, G., Xu, Z., Wang, F. et al. Extraction, characterization, and biological activities of exopolysaccharides from plant root soil fungus Fusarium merismoides A6. Braz J Microbiol 54, 199–211 (2023). https://doi.org/10.1007/s42770-022-00842-x

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