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Molecular characterization of KPC-2-producing Klebsiella pneumoniae ST258 isolated from bovine mastitis

  • Veterinary Microbiology - Short Communication
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Abstract

Bovine mastitis, an inflammation of the mammary gland of dairy cattle, is the most prevalent disease causing economically important losses, reduced milk production, early culling, veterinary expenses, and higher death rates. Bovine mastitis infections are the main cause for the use of antibiotics; however, the emergence of multidrug-resistant bacteria and the poor or nil response to antibiotics has become a critical global health problem. The goal of this study was the characterization of bacterial infections associated with clinical bovine mastitis. All the isolates were multidrug-resistant and were negative for the production of extended spectrum β-lactamases. However, all isolates were identified as carbapenemase-producing organisms by the Carba NP test. The carbapenemase identified was the product of the KPC-2 gene. The isolates were identified as Klebsiella pneumoniae and contained virulence genes for fimbriae, lipopolysaccharides, nitrogen starvation genes, and siderophores. Sixty-nine percent of the KPC-2-producing isolates had the same plasmid profile, although the genetic mobilization of resistance by bacterial conjugation was unsuccessful. The carbapenemase corresponded to the plasmid-borne KPC-2 gene identified by Southern blot hybridization. The assay showed a positive signal in the 90 kb (69% of the isolates), 165 kb (31% of the isolates), and 130 kb (6% of the isolates) plasmids. The IncFIIy and IncFIIk replicons were detected among these K. pneumoniae isolates. The PFGE and MLST analysis showed that all of the isolates are comprised by two clones (A and B) belonging to Sequence Type 258. This is the first report of K. pneumoniae producing carbapenemase KPC-2 isolated from bovine mastitis.

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Acknowledgements

We thank Dr. Michael Dunn form the Center for Genomic Science-UNAM for reviewing the manuscript.

Funding

The present study was supported by grants 256988 and 256927 from SEP-CONACyT (Secretaría de Educación Pública-Consejo Nacional de Ciencia y Tecnología).

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Author notes

  1. Jesús Silva-Sanchez and Humberto Barrios-Camacho contributed equally to this work.

Authors and Affiliations

  1. Laboratorio de Resistencia Bacteriana, Centro de Investigación Sobre Enfermedades Infecciosas (CISEI), Instituto Nacional de Salud Pública (INSP), Av. Universidad # 655, Col. Santa María Ahuacatitlán. C.P, 62100, Cuernavaca, Morelos, México

    Jesús Silva-Sanchez, Humberto Barrios-Camacho, Emmanuel Hernández-Rodriguez, Josefina Duran-Bedolla, Alejandro Sanchez-Perez & Ulises Garza-Ramos

  2. Departamento de Patología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Coyoacán, 04510, Ciudad de México, México

    Luary C. Martínez-Chavarría

  3. Laboratorio de Bacteriología Intestinal, Hospital Infantil de México, Dr. Federico Gómez, Cuauhtémoc, 06720, Ciudad de México, México

    Juan Xicohtencatl-Cortes

  4. Departamento Ecología de Agentes Patógenos, Hospital General “Dr. Manuel Gea González”, Calzada de Tlalpan 4800, 14080. Tlalpan, Cd de Mexico, Mexico

    Rigoberto Hernández-Castro

Authors
  1. Jesús Silva-Sanchez
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  2. Humberto Barrios-Camacho
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  3. Emmanuel Hernández-Rodriguez
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  4. Josefina Duran-Bedolla
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  5. Alejandro Sanchez-Perez
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  6. Luary C. Martínez-Chavarría
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  8. Rigoberto Hernández-Castro
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  9. Ulises Garza-Ramos
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Correspondence to Rigoberto Hernández-Castro or Ulises Garza-Ramos.

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Silva-Sanchez, J., Barrios-Camacho, H., Hernández-Rodriguez, E. et al. Molecular characterization of KPC-2-producing Klebsiella pneumoniae ST258 isolated from bovine mastitis. Braz J Microbiol 52, 1029–1036 (2021). https://doi.org/10.1007/s42770-021-00445-y

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