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Accuracy of PCR universal primer for methicillin-resistant Staphylococcus and comparison of different phenotypic screening assays

  • Veterinary Microbiology - Short Communication
  • Published:
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Abstract

Characterization of methicillin-resistant Staphylococcus (MRS) is a continuous challenge at diagnostic laboratories. The phenotypic methods present heterogeneous results and the occurrence of variants of mecA gene turned this goal even more challenging to achieve. The present study provided an accurate and highly discriminatory screening tool for MRS, improving its detection.

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Acknowledgments

We thank Dr. C. M. Logue and Dr. L.K. Nolan for supporting this study at Veterinary Microbiology Research Institute, Iowa State University.

Funding

Foundation for Research Support of the State of Rio de Janeiro, scholarship protocol number E26/200.101/2016, and National Council for Scientific and Technological Development supported this work.

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Authors and Affiliations

  1. Veterinary Microbiology and Immunology Department, Federal Rural University of Rio de Janeiro, Rio de Janeiro, Brazil

    Dayanne A. de Melo, Bianca da S. Soares, Cássia C. da Motta, Felipe C. Dubenczuck, Shana de O. Coelho, Irene da S. Coelho & Miliane M. S. de Souza

  2. Department of Population Health, University of Georgia, College of Veterinary Medicine, Athens, GA, USA

    Nicolle L. Barbieri & Catherine M. Logue

Authors
  1. Dayanne A. de Melo
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  2. Bianca da S. Soares
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  3. Cássia C. da Motta
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  4. Felipe C. Dubenczuck
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  5. Nicolle L. Barbieri
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  6. Catherine M. Logue
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  7. Shana de O. Coelho
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  8. Irene da S. Coelho
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  9. Miliane M. S. de Souza
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Corresponding author

Correspondence to Miliane M. S. de Souza.

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The authors declare that they have no conflicts of interest.

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Responsible Editor: Marina Baquerizo Martinez.

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de Melo, D.A., da S. Soares, B., da Motta, C.C. et al. Accuracy of PCR universal primer for methicillin-resistant Staphylococcus and comparison of different phenotypic screening assays. Braz J Microbiol 51, 403–407 (2020). https://doi.org/10.1007/s42770-019-00171-6

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  • DOI: https://doi.org/10.1007/s42770-019-00171-6

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