Abstract
Indiscriminate use of chemicals in agriculture impacts soil properties, significantly compromising microbial diversity. Therefore, integrating eco-friendly strategies with minimal adverse impact on soil microflora and increasing crop productivity are essential for sustainable agriculture. The study was designed to understand the impact of different farming practices on the rhizospheric bacterial populations involved in nitrogen and phosphorus cycles, soil nutrient contents, and the uptake of nutrients by plants. A 3-year field experiment was set up in a randomized block pattern with pigeonpea-wheat cropping system (PWCS). Conventional and organic farming management practices were selected with two sets of organic amendments (ORG1: farmyard manure, ORG2: leaf compost + crop residue), three sets of conventional treatment [CON1: farmyard manure + 50% NPK (nitrogen, phosphorus, and potassium), CON2: leaf compost + crop residue + 50% NPK, and CON3: 100% NPK], and control (C). Plants and soil were sampled at the harvest stage of the crops for three consecutive cropping seasons to assess the soil and plant nutrient contents, and N and P cycling bacterial guilds in the rhizospheres. The total NPK uptake by plants was higher in conventional treatments than in organic treatments for both the crops, compared to the control treatment. Bacterial genes involved in the N and P cycles positively correlated with organic farming practice in both crops. Overall, agricultural management strategies had a significant impact on PWCS. The amoA gene was the most sensitive marker contributing to the variation in the abundance of the functional bacterial community in response to agri-management practices. Soil and plant NPK content showed a significant positive correlation in the case of the pigeonpea crop. The study showed the positive effect of different organic amendments on rhizospheric bacterial guilds. The combination of organic amendments with reduced chemical fertilizers intensifies the functioning of the N and P cycles. Organic amendments and reduced chemical fertilizers in PWCS offer a promising avenue for improving nutrient cycling, enhancing soil health, and ultimately bolstering crop productivity.
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References
Bauhus J, Khanna PK (1994) Carbon and nitrogen turnover in two acid forest soils of southeast Australia as affected by phosphorus addition and drying and rewetting cycles. Biol Fertil Soils 17:212–218. https://doi.org/10.1007/BF00336325
Bhardwaj Y, Reddy B, Dubey SK (2023) Organic farming favors phoD-harboring rhizospheric bacterial community and alkaline phosphatase activity in tropical agroecosystem. Plants 12:1068. https://doi.org/10.3390/plants12051068
Bhattacharyya R, Kundu S, Prakash V, Gupta HS (2008) Sustainability under combined application of mineral and organic fertilizers in a rainfed soybean–wheat system of the Indian Himalayas. Eur J Agron 28:33–46. https://doi.org/10.1016/j.eja.2007.04.006
Bindraban PS, Dimkpa C, Nagarajan L, Roy A, Rabbinge R (2015) Revisiting fertilizers and fertilization strategies for improved nutrient uptake by plants. Biol Fertil Soils 51:897–911. https://doi.org/10.1007/s00374-015-1039-7
Bokhtiar SM, Sakurai K (2005) Effects of organic manure and chemical fertilizer on soil fertility and productivity of plant and ratoon crops of sugarcane. Arch Agron Soil Sci 51:325–334. https://doi.org/10.1080/03650340500098006
Bouyoucos CJ (1962) Hydrometer method improved for making particle size analysis of soil. Agron J 54:464–465. https://doi.org/10.2134/agronj1962.00021962005400050028x
Bowles TM, Acosta-Martínez V, Calderón F, Jackson LE (2014) Soil enzyme activities, microbial communities, and carbon and nitrogen availability in organic agroecosystems across an intensively-managed agricultural landscape. Soil Biol Biochem 68:252–262. https://doi.org/10.1016/j.soilbio.2013.10.004
Casida L, Klein D, Santoro T (1964) Soil dehydrogenase activity. Soil Sci 98:371–376. https://doi.org/10.1097/00010694-196412000-00004
Chen Z, Luo X, Hu R, Wu M, Wu J, Wei W (2010) Impact of long-term fertilization on the composition of denitrifier communities based on nitrite reductase analyses in a paddy soil. Microb Ecol 60:850–861. https://doi.org/10.1016/S2095-3119(14)60784-X
Chen Z, Lin S, Yao Z, Zheng X, Gschwendtner S, Schloter M, Dannenmann M et al (2018) Enhanced nitrogen cycling and N2O loss in water-saving ground cover rice production systems (GCRPS). Soil Biol Biochem 121:77–86. https://doi.org/10.1016/j.soilbio.2018.02.015
Dai Z, Liu G, Chen H, Chen C, Wang J, Ai S, Xu J et al (2020) Long-term nutrient inputs shift soil microbial functional profiles of phosphorus cycling in diverse agroecosystems. ISME J 14:757–770. https://doi.org/10.1038/s41396-019-0567-9
Ducousso-Détrez A, Fontaine J, Lounès-HadjSahraoui A, Hijri M (2022) Diversity of phosphate chemical forms in soils and their contributions on soil microbial community structure changes. Microorganisms 10:609. https://doi.org/10.3390/microorganisms10030609
Dutta S, Pal R, Chakraborty A, Chakrabarti K (2003) Influence of integrated plant nutrient supply system on soil quality restoration in a red and laterite soil. Arch Agron Soil Sci 49:631–637. https://doi.org/10.1080/03650340310001599722
Elhaissoufi W, Ghoulam C, Barakat A, Zeroual Y, Bargaz A (2022) Phosphate bacterial solubilization: a key rhizosphere driving force enabling higher P use efficiency and crop productivity. J Adv Res 38:13–28. https://doi.org/10.1016/j.jare.2021.08.014
Fageria NK, Oliveira JP (2014) Nitrogen, phosphorus and potassium interactions in upland rice. J Plant Nutr 37:1586–1600. https://doi.org/10.1080/01904167.2014.920362
FAO (2019) World fertilizer trends and outlook to 2022. Rome. http://www.fao.org/3/ca6746en/ca6746en.pdf
Gai X, Liu H, Liu J, Zhai L, Yang B, Wu S, Wang H et al (2018) Long-term benefits of combining chemical fertilizer and manure applications on crop yields and soil carbon and nitrogen stocks in North China Plain. Agric Water Manag 208:384–392. https://doi.org/10.1016/j.agwat.2018.07.002
Garcıa-Gil JC, Plaza C, Soler-Rovira P, Polo A (2000) Long-term effects of municipal solid waste compost application on soil enzyme activities and microbial biomass. Soil Biol Biochem 32:1907–1913. https://doi.org/10.1016/S0038-0717(00)00165-6
García-Orenes F, Roldán A, Mataix-Solera J, Cerdà A, Campoy M, Arcenegui V, Caravaca F et al (2012) Soil structural stability and erosion rates influenced by agricultural management practices in a semi-arid Mediterranean agro-ecosystem. Soil Use Manag 28:571–579. https://doi.org/10.1111/j.1475-2743.2012.00451.x
Gryta A, Frąc M, Oszust K (2019) Community shift in structure and functions across soil profile in response to organic waste and mineral fertilization strategies. Appl Soil Ecol 143:55–60
Gupta G, Dhar S, Kumar A, Choudhary AK, Dass A, Sharma VK, Shukla L, Upadhyay PK, Das A, Jinger D, Rajpoot SK, Sannagoudar MS, Kumar A, Bhupenchandra I, Tyagi V, Joshi E, Kumar K, Dwivedi P, Rajawat MVS (2022) Microbes-mediated integrated nutrient management for improved rhizo-modulation, pigeonpea productivity, and soil bio-fertility in a semi-arid agro-ecology. Front Microbiol 13:924407. https://doi.org/10.3389/fmicb.2022.924407
Hai B, Diallo NH, Sall S, Haesler F, Schauss K, Bonzi M, Schloter M et al (2009) Quantification of key genes steering the microbial nitrogen cycle in the rhizosphere of sorghum cultivars in tropical agroecosystems. Appl Environ Microbiol 75:4993–5000. https://doi.org/10.1128/AEM.02917-08
Hanway JJ, Heidel H (1952) Soil analysis methods as used in Iowa State College Soil Testing Laboratory, Bulletin 57. Iowa State College of Agriculture, Iowa, USA, p 131
Hao J, Feng Y, Wang X, Yu Q, Zhang F, Yang G, Ren C et al (2022) Soil microbial nitrogen-cycling gene abundances in response to crop diversification: a meta-analysis. Sci Total Environ 838:156621. https://doi.org/10.1016/j.scitotenv.2022.156621
Haridasan M, ME M, Victoria RL, Richey JR (2001) Nutrient cycling as a function of landscape and biotic characteristics. The Cerrados of Central Brazil. In: The biochemistry of the Amazon Basin, Oxford University Press, New York, 68-83
Hartmann M, Frey B, Mayer J, Mäder P, Widmer F (2015) Distinct soil microbial diversity under long-term organic and conventional farming. ISME J 9:1177–1194. https://doi.org/10.1038/ismej.2014.210
Havlin JL (2020) Soil: fertility and nutrient management. In Landscape and land capacity (pp. 251–265). CRC Press.
Hu Y, Xia Y, Sun Q, Liu K, Chen X, Ge T, Su Y et al (2018) Effects of long-term fertilization on phoD-harboring bacterial community in Karst soils. Sci Total Environ 628:53–63. https://doi.org/10.1016/j.scitotenv.2018.01.314
Hu J, Jin VL, Konkel JY, Schaeffer SM, Schneider LG, DeBruyn JM (2021) Soil health management enhances microbial nitrogen cycling capacity and activity. Msphere 6:10–1128
Jackson ML (1973) Soil chemical analysis. New Delhi, Prentice Hall of India Private Limited, p 187
Knapp S, van der Heijden MG (2018) A global meta-analysis of yield stability in organic and conservation agriculture. Nat Commun 9:3632. https://doi.org/10.1038/s41467-018-05956-1
Kong D, Ren C, Yang G, Liu N, Sun J, Zhu J, Ren G, Feng Y (2022) Long-term wheat-soybean rotation and the effect of straw retention on the soil nutrition content and bacterial community. Agronomy 12:2126
Kumar A, Rana KS, Choudhary AK, Bana RS, Sharma VK, Prasad S, Gupta G, Choudhary M, Pradhan A, Rajpoot SK, Kumar A, Kumar A, Tyagi V (2021) Energy budgeting and carbon footprints of zero-tilled pigeonpea–wheat cropping system under sole or dual crop basis residue mulching and Zn-fertilization in a semiarid agro-ecology. Energy 231:120862. https://doi.org/10.1016/j.energy.2021.120862
Lagos LM, Acuña JJ, Maruyama F, Ogram A, de la Luz Mora M, Jorquera MA (2016) Effect of phosphorus addition on total and alkaline phosphomonoesterase-harboring bacterial populations in ryegrass rhizosphere microsites. Biol Fertil Soils 52:1007–19
Lazcano C, Gómez-Brandón M, Revilla P, Domínguez J (2013) Short-term effects of organic and inorganic fertilizers on soil microbial community structure and function: a field study with sweet corn. Biol Fertil Soils 49:723–733. https://doi.org/10.1007/s00374-012-0761-7
Li F, Chen L, Zhang J, Yin J, Huang S (2017) Bacterial community structure after long-term organic and inorganic fertilization reveals important associations between soil nutrients and specific taxa involved in nutrient transformations. Front Microbiol 8:187. https://doi.org/10.3389/fmicb.2017.00187
Li Y, Chen X, Tang C, Zeng M, Li S, Ling Q, Liu K, Ma J, Tang S, Yu F (2023) Variations on the diazotrophic community in the rhizosphere soil of three dominant plant species in a lead–zinc mine area. Plant and Soil 28:1–21
Liu Z, Guo Q, Feng Z, Liu Z, Li H, Sun Y, Lai H (2020) Long-term organic fertilization improves the productivity of kiwifruit (Actinidia chinensis Planch.) through increasing rhizosphere microbial diversity and network complexity. Appl Soil Ecol 147:103426. https://doi.org/10.1016/j.apsoil.2019.103426
Luo G, Xue C, Jiang Q, Xiao Y, Zhang F, Guo S, Ling N (2020) Soil carbon, nitrogen, and phosphorus cycling microbial populations and their resistance to global change depend on soil C: N: P stoichiometry. Msystems 5:e00162-e220. https://doi.org/10.1128/mSystems.00162-20
Luttikholt LW (2007) Principles of organic agriculture as formulated by the International Federation of Organic Agriculture Movements. NJAS - Wagening J Life Sci 54:347–360. https://doi.org/10.1016/S1573-5214(07)80008-X
Meyer JB, Frapolli M, Keel C, Maurhofer M (2011) Pyrroloquinoline quinone biosynthesis gene pqqC, a novel molecular marker for studying the phylogeny and diversity of phosphate-solubilizing pseudomonads. Appl Environ Microbiol 77:7345–7354. https://doi.org/10.1128/AEM.05434-11
Nieves-Cordones M, Rubio F, Santa-María GE (2020) Nutrient use-efficiency in plants: an integrative approach. Front Plant Sci 11:623976. https://doi.org/10.3389/fpls.2020.623976
Nunan N, Morgan MA, Herlihy M (1998) Ultraviolet absorbance (280 nm) of compounds released from soil during chloroform fumigation as an estimate of microbial biomass. Soil Biol Biochem 30:1599–1603. https://doi.org/10.1016/S0038-0717(97)00226-5
Olsen SR, Cole CV, Watanabe FS, Dean LA (1954) Estimation of available phosphorus in soils by extraction with sodium bicarbonate (USDA Circular No. 939, pp 1–19). US Government Printing Office, Washington DC, USA.
Olsen SR, Sommers LE (1982) Phosphorus. Methods Soil Anal Part 2:403–430
Ouyang Y, Evans SE, Friesen ML, Tiemann LK (2018) Effect of nitrogen fertilization on the abundance of nitrogen cycling genes in agricultural soils: a meta-analysis of field studies. Soil Biol Biochem 127:71–78. https://doi.org/10.1016/j.soilbio.2018.08.024
Ouyang Y, Reeve JR, Norton JM (2018) Soil enzyme activities and abundance of microbial functional genes involved in nitrogen transformations in an organic farming system. Biol Fertil Soils 54:437–450. https://doi.org/10.1007/s00374-018-1272-y
Pahalvi HN, Rafiya L, Rashid S, Nisar B, Kamili AN (2021) Chemical fertilizers and their impact on soil health. In Microbiota and biofertilizers, Vol 2 (pp. 1–20). Springer, Cham. https://doi.org/10.1007/978-3-030-61010-4_1
Pajares S, Bohannan BJ (2016) Ecology of nitrogen fixing, nitrifying, and denitrifying microorganisms in tropical forest soils. Front Microbiol 7:1045. https://doi.org/10.3389/fmicb.2016.01045
Piper CS (1965) Soil and plant analysis. The University of Adelaide Press, Adelaide, Australia, p 355
Piper DW, Fenton BH (1965) pH stability and activity curves of pepsin with special reference to their clinical importance. Gut 6:506
Prasad R (1965) Determination of potentially available nitrogen in soils-a rapid procedure. Plant Soil 261–264. https://doi.org/10.1007/BF01358352
Qaswar M, Jing H, Ahmed W, Dongchu L, Shujun L, Lu Z, Huimin Z (2020) Yield sustainability, soil organic carbon sequestration and nutrients balance under long-term combined application of manure and inorganic fertilizers in acidic paddy soil. Soil Tillage Res 198:104569. https://doi.org/10.1016/j.still.2019.104569
Rana KS, Choudhary AK, Sepat S, Bana RS, Dass A (2014) Methodological and analytical agronomy. New Delhi, India, Post Graduate School IARI, p 276
Richardson AE, Simpson RJ (2011) Soil microorganisms mediating phosphorus availability update on microbial phosphorus. Plant Physiol 156:989–996. https://doi.org/10.1104/pp.111.175448
Richardson AE, Lynch JP, Ryan PR, Delhaize E, Smith FA, Smith SE, Simpson RJ (2011) Plant and microbial strategies to improve the phosphorus efficiency of agriculture. Plant Soil 349:121–156. https://doi.org/10.1007/s11104-011-0950-4
Rose A, Padovan A, Christian K, van de Kamp J, Kaestli M, Tsoukalis S, Gibb K (2021) The diversity of nitrogen-cycling microbial genes in a waste stabilization pond reveals changes over space and time that is uncoupled to changing nitrogen chemistry. Microb Ecol 81:1029–1041. https://doi.org/10.1007/s00248-020-01639-x
Rosenkranz S, Wilcke W, Eisenhauer N, Oelmann Y (2012) Net ammonification as influenced by plant diversity in experimental grasslands. Soil Biol Biochem 48:78–87
Saba T, Liu W, Wang J, Saleem F, Kang X, Hui W, Li H (2022) Effects of organic supplementation to reduced rates of chemical fertilization on soil fertility of Zanthoxylum armatum. Dendrobiology 87. https://doi.org/10.12657/denbio.087.009
Schloter M, Nannipieri P, Sørensen SJ, van Elsas JD (2018) Microbial indicators for soil quality. Biol Fertil Soils 54:1–10. https://doi.org/10.1007/s00374-017-1248-3
Schoebitz M, Castillo D, Jorquera M, Roldan A (2020) Responses of microbiological soil properties to intercropping at different planting densities in an acidic Andisol. Agronomy 10:781
Shahid M, Nayak AK, Puree C, Tripathi R, Lal B, Gautam P, Bhattacharyya P, Mohanty S, Kumar A, Panda BB, Kumar U (2017) Carbon and nitrogen fractions and stocks under 41 years of chemical and organic fertilization in a sub-humid tropical rice soil. Soil tillage res 170:136–46
Sharma S, Mehta R, Gupta R, Schloter M (2012) Improved protocol for the extraction of bacterial mRNA from soils. J Microbiol Methods 91:62–64. https://doi.org/10.1016/j.mimet.2012.07.016
Shrivas VL, Choudhary AK, Shidture S, Rambia A, Hariprasad P, Sharma A, Sharma S (2023) Organic amendments modulate the crop yield and rhizospheric bacterial community diversity: a 3-year field study with Cajanus cajan. Int Microbiol 27:1–4
Singh U, Choudhary AK, Sharma S (2021) Agricultural practices modulate the bacterial communities, and nitrogen cycling bacterial guild in rhizosphere: Field experiment with soybean. J Sci Food Agric 101:2687–2695. https://doi.org/10.1002/jsfa.10893
Sintes E, Bergauer K, De Corte D, Yokokawa T, Herndl GJ (2013) Archaeal amoA gene diversity points to distinct biogeography of ammonia-oxidizing Crenarchaeota in the ocean. Environ Microbiol 15:1647–58
Smith OM, Cohen AL, Rieser CJ, Davis AG, Taylor JM, Adesanya AW, Crowder DW (2019) Organic farming provides reliable environmental benefits but increases variability in crop yields: a global meta-analysis. Front Sustain Food Syst 3:82. https://doi.org/10.3389/fsufs.2019.00082
Subbiah BV, Asija GL (1956) A rapid procedure for the determination of available-N in soils. Curr Sci 25:259–260
Sun R, Guo X, Wang D, Chu H (2015) Effects of long-term application of chemical and organic fertilizers on the abundance of microbial communities involved in the nitrogen cycle. Appl Soil Ecol 95:171–178. https://doi.org/10.1016/j.apsoil.2015.06.010
Tabatabai MA, Bremner JM (1969) Use of p-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Biol Biochem 1:254–260
Tripathi S, Srivastava P, Devi RS, Bhadouria R (2020) Influence of synthetic fertilizers and pesticides on soil health and soil microbiology. In Agrochemicals detection, treatment and remediation (pp. 25–54). Butterworth-Heinemann. https://doi.org/10.1016/b978-0-08-103017-2.00002-7
Veihmeyer FJ, Hendrickson A (1948) Soil density and root penetration. Soil Sci 65:487–494
Walkley A, Black CA (1934) An examination of the Dagtjareff (wet acid) method for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Sci 37:29–38
Wan W, Li X, Han S, Wang L, Luo X, Chen W, Huang Q (2020) Soil aggregate fractionation and phosphorus fraction driven by long-term fertilization regimes affect the abundance and composition of P-cycling-related bacteria. Soil Tillage Res 196:104475. https://doi.org/10.1016/j.still.2019.104475
Wang Q, Wang C, Yu W, Turak A, Chen D, Huang Y, Huang Z (2018) Effects of nitrogen and phosphorus inputs on soil bacterial abundance, diversity, and community composition in Chinese fir plantations. Front Microbiol 9:1543. https://doi.org/10.3389/fmicb.2018.01543
Wang Y, Zhu Y, Zhang S, Wang Y (2018) What could promote farmers to replace chemical fertilizers with organic fertilizers? J Clean Prod 199:882–890. https://doi.org/10.1016/j.jclepro.2018.07.222
Wang F, Kertesz MA, Feng G (2019) Phosphorus forms affect the hyphosphere bacterial community involved in soil organic phosphorus turnover. Mycorrhiza 29:351–362. https://doi.org/10.1007/s00572-019-00896-0
Wang J, Chen Z, Xu C, Elrys AS, Shen F, Cheng Y, Chang SX (2021) Organic amendment enhanced microbial nitrate immobilization with negligible denitrification nitrogen loss in an upland soil. Environ Pollut 288:117721. https://doi.org/10.1016/j.envpol.2021.117721
Warton DI, Wright ST, Wang Y (2012) Distance-based multivariate analyses confound location and dispersion effects. Methods Ecol Evol 3:89–101
Wendling M, Büchi L, Amossé C, Sinaj S, Walter A, Charles R (2016) Influence of root and leaf traits on the uptake of nutrients in cover crops. Plant Soil 409:419–434. https://doi.org/10.1007/s11104-016-2974-2
Wu X, Peng J, Liu P, Bei Q, Rensing C, Li Y, Yuan H, Liesack W, Zhang F, Cui Z (2021) Metagenomic insights into nitrogen and phosphorus cycling at the soil aggregate scale driven by organic material amendments. Sci Total Environ 785:147329. https://doi.org/10.1016/j.scitotenv.2021.147329
Wu X, Cui Z, Peng J et al (2022) Genome-resolved metagenomics identifies the particular genetic traits of phosphate-solubilizing bacteria in agricultural soil. ISME Commun 2:17. https://doi.org/10.1038/s43705-022-00100-z
Wu X, Liu Y, Shang Y et al (2022) Peat-vermiculite alters microbiota composition towards increased soil fertility and crop productivity. Plant Soil 470:21–34. https://doi.org/10.1007/s11104-021-04851-x
Zeng J, Liu X, Song L, Lin X, Zhang H, Shen C, Chu H (2016) Nitrogen fertilization directly affects soil bacterial diversity and indirectly affects bacterial community composition. Soil Biol Biochem 92:41–49. https://doi.org/10.1016/j.soilbio.2015.09.018
Zhang X, Zhang R, Gao J, Wang X, Fan F, Ma X, Deng Y (2017) Thirty-one years of rice-rice-green manure rotations shape the rhizosphere microbial community and enrich beneficial bacteria. Soil Biol Biochem 104:208–217. https://doi.org/10.1016/j.soilbio.2016.10.023
Zhang M, Zhang X, Zhang L, Zeng L, Liu Y, Wang X, Ai C (2021) The stronger impact of inorganic nitrogen fertilization on soil bacterial community than organic fertilization in short-term condition. Geoderma 382:114752. https://doi.org/10.1016/j.geoderma.2020.114752
Zhang Q, Zhao W, Zhou Z, Huang G, Wang X, Han Q, Liu G (2022) The application of mixed organic and inorganic fertilizers drives soil nutrient and bacterial community changes in teak plantations. Microorganisms 10:958. https://doi.org/10.3390/microorganisms10050958
Zhou J, Jiang X, Wei D, Zhao B, Ma M, Chen S, Li J et al (2017) Consistent effects of nitrogen fertilization on soil bacterial communities in black soils for two crop seasons in China. Sci Rep 7:3267. https://doi.org/10.1038/s41598-017-03539-6
Zhou G, Gao S, Lu Y, Liao Y, Nie J, Cao W (2020) Co-incorporation of green manure and rice straw improves rice production, soil chemical, biochemical and microbiological properties in a typical paddy field in southern China. Soil Tillage Res 197:104499. https://doi.org/10.1016/j.still.2019.104499
Acknowledgements
VLS acknowledges the fellowship received from IIT Delhi. The authors wish to thank Dr. Amit Das, Department of Biochemical Engineering and Biotechnology, IIT Delhi, for his help with statistical analysis.
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This research was supported by the Department of Biotechnology, Government of India (BT/PR27680/BCE/8/1434/2018).
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Shrivas, V.L., Choudhary, A.K., Dass, A. et al. Impact of Different Farming Practices on Soil Nutrients and Functional Bacterial Guilds in Pigeonpea-Wheat Crop Rotation. J Soil Sci Plant Nutr 24, 684–699 (2024). https://doi.org/10.1007/s42729-023-01575-y
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DOI: https://doi.org/10.1007/s42729-023-01575-y