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Semi-field evaluation of bacterial bio-pesticide Streptomyces indiaensis (LMG19961) susceptibility against Dengue Vector: Aedes aegypti (L.)

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Abstract

Dengue is the most important arthropod borne viral disease of community health significance which is regarded as most prevalent mosquito born disease. Development of resistance against commonly used insecticides against vectors has posed a serious challenge in vector control programmes in many countries including India. Till date, many strategies have been used in vector control. Entomo-pathogens (Bacterial, Viral, Fungal and many Nematodes) have become classical biological agents for many vector control programmes. But lack of proper field trial generally causes a major setback in commercialization of many effective easily available products. The present study is an attempt to evaluate the potentiality of a newly discovered bacterial isolate, S. indiaensis (strain LMG19961) against a deadly vector of dengue i.e., Ae. aegypti (L.) larvae in semi-field as well as in laboratory conditions. In the laboratory, larvicidal activity of the S. indiaensis (strain LMG 19961) was evaluated as per the method recommended by World Health Organization (2005). The semi-field bioassay was carried out to assess the efficacy of S. indiaensis (strain LMG 19961) in the semi-field environment to control dengue vector Ae. aegypti (L.) according to protocol of Bukhari et al. (2011). S. indiaensis (strain LMG19961) has been proved highly effective against dengue mosquitoes in the laboratory bioassay with LC50 and LC90 values of 1.979 & 3.561 cfu/ml respectively and 94.44 percent mortality at 3.5 × 14x106 cfu/ml concentration after maximum treatment time of 48 h. Further larvicidal activity of S. indiaensis (strain LMG19961) was performed in semi-field conditions. In the semi-field, pupal emergence at 1.0, 2.5, 5.0 × 2.7 × 103 cfu/gm was 5.33, 2.67, and 1.33 as compared to control group of 49.67 percent. Similarly adult emergence was also observed, the adult emergence at 1.0, 2.5, 5.0 × 2.7 × 103 cfu/gm were 2.33, 1.67, 0.67 respectively and in control adult emergence was 49.67 percent. The present study confirmed that S. indiaensis (strain LMG19961) has potential for control of larval population in the field as well as in laboratory. Semi-field evaluation of this newly isolated bacterial strain opens a new era in bio-control of Aedes mosquitoes for dengue control.

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Availability of data and materials

All data generated and analyzed for the current study are presented in this manuscript, and the corresponding author has no objection to the availability of data and materials.

Abbreviations

S. indiaensis :

Streptomyces indiaensis

Ae. Aegypti :

Aedes aegypti

0C:

Degree Centigrade

Lbs:

Pounds

min:

Minutes

ml:

Milliliter

LC:

Lethal Concentration

cfu:

Colony Forming Unit

gm:

Gram

cm:

Centimeter

ANOVA:

Analysis of Variance

df:

Degree of Freedom

N:

Number of replicate

F:

ANOVA Coefficient

SD:

Standard Deviation

NS:

Non-Significant

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Acknowledgements

The authors are grateful to the Professor and Head, Department of Zoology, University College of Science, Mohanlal Sukhadia University, Udaipur, Rajasthan, India for the laboratory facilities provided for the present investigation. We acknowledge the Research Scholars of Laboratory of Public Health Entomology, Zoology Department of Mohanlal Sukhadia University, Udaipur, Rajasthan for their Help and Support.

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Not Applicable.

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Authors and Affiliations

  1. Research Associate, Laboratory of Public Health Entomology, Department of Zoology, Mohanlal Sukhadia University, 313001, Udaipur, Rajasthan, India

    Ashok Kumar

  2. Prof., Laboratory Incharge and Head, Laboratory of Public Health Entomology, Department of Zoology, Mohanlal Sukhadia University, 313001, Udaipur, Rajasthan, India

    Arti Prasad

Authors
  1. Ashok Kumar
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Contributions

AK: Collected soil samples, isolated bacterial isolate, performed laboratory and semi-field/field trials, analyzed and interpreted the data of the work and prepared the original manuscript. AP: designed and guided all protocols, the laboratory as well as the semi-field trials, reviewed and edited the writing. The authors read and approved the final manuscript.

Corresponding author

Correspondence to Ashok Kumar.

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Not applicable. The study was conducted using local isolates of bacterial antagonists that are abundant in the ecosystem hence does not need ethical approval.

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The authors agreed to publish this paper. The data have not been published partially or completely in any other journal.

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The Authors declare that they have no recognized competing financial interests or individual relationship that could have appeared to influence the work reported in this paper.

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Kumar, A., Prasad, A. Semi-field evaluation of bacterial bio-pesticide Streptomyces indiaensis (LMG19961) susceptibility against Dengue Vector: Aedes aegypti (L.). Int J Trop Insect Sci 44, 63–70 (2024). https://doi.org/10.1007/s42690-023-01131-2

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