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Effect of various chemical solutions on micropropagation efficiency, antioxidant activities and clonal fidelity analysis of in vitro developed plantlets in pomegranate (Punica granatum L.) using SSR markers

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Abstract

This study aimed to develop an effective in vitro protocol for micropropagation of pomegranate (Punica granatum L.) var. Bhagwa using nodal explants. To induce shoot growth and cultivating the explants, Murashige and Skoog’s media were supplemented with benzyl amino purine (BAP), sodium nitroprusside (SNP), an approach demonstrated and experimented for the first time on Pomegranate and kinetin (Kn) in various concentrations and combinations. In the MS medium fortified with 1.5 mg/l BAP, 1.5 mg/l SNP, and 1.5 mg/l Kn, maximum shoot length, shoot per explants, and regeneration % were noted. For root induction, uniform micro shoots were separated and then placed in the rooting media (Murashige and Skoog medium at half strength), supplemented with different ratios of NAA and SNP with 2 mg/L NAA and 0.3 mg/L SNP yielding the highest culture rooting, number of roots, and root length. The described methodology permits the development of multiple pomegranate plants that have been micro propagated. Superoxide dismutase, catalase, and guaiacol peroxidase activities revealed the antioxidant defence mechanism of micro propagated pomegranate micro shoots, which increased shoot development and the number of shoots per explant. Using simple sequence repeat (SSR) markers, the mother plants and proliferating plantlets were checked for genetic integrity; the results showed that all of the plants were the same. The results of this study could be used commercially in the future to produce high-quality pomegranate planting material.

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Abbreviations

BAP/BA:

Banzyl amino purine/ Benzyl adenine

NAA:

Napthaline acetic acid

SNP:

Sodium Nitro Prusside

MS:

Murashige and Skoog

Kn:

Kinetin

SOD:

Superoxide dismutase

CAT:

Catalase

GPO:

Guaiacol peroxidise

CTAB:

Cetyl Triethyl Ammonium Bromide

CRD:

Completely randomized design

ANOVA:

Analysis of variance

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Acknowledgements

Authors are thankful to Director Research, SVPUAT for providing all the necessary support for conducting the present study.

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Authors and Affiliations

  1. College of Horticulture, Sardar Vallabhbhai Patel University of Agriculture & Technology, 250110, Meerut, UP, India

    Devendra Pal, Mukesh Kumar, Chetan Chauhan, Arvind Kumar & V. Rakesh Sharma

  2. College of Agriculture, Sardar Vallabhbhai Patel University of Agriculture & Technology, 250110, Meerut, UP, India

    Manoj Kumar Yadav & Ujjwal Sirohi

  3. Department of Chemistry, Meerut College, 250002, Meerut, UP, India

    Veena Chaudhary

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  1. Devendra Pal
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  2. Mukesh Kumar
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Correspondence to Mukesh Kumar.

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Pal, D., Kumar, M., Yadav, M.K. et al. Effect of various chemical solutions on micropropagation efficiency, antioxidant activities and clonal fidelity analysis of in vitro developed plantlets in pomegranate (Punica granatum L.) using SSR markers. Vegetos (2024). https://doi.org/10.1007/s42535-024-00846-y

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