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Purification and functional characterization of lectin from Chenopodium album

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Abstract

Chenopodium album L. is a nutritious and edible vegetable with various health benefits. It has been traditionally used for the treatment of several diseases due to its acclaimed medicinal properties. In this study, lectin from seeds of Chenopodium album (CaLec) was purified through anion chromatography of ~ 54 kDa. SDS-PAGE analysis revealed heteropolypeptide nature of CaLec consisting of ~ 35 kDa and ~ 19 kDa subunits. CaLec exhibited inhibition of two pathogenic bacterial strains, Staphylococcus aureus and Escherichia coli. This inhibitory effect was more prominent for S. aureus as compared to E. coli. Furthermore, the anti-tumor effect of purified CaLec was tested in Hepatoma HepG2 cells by measuring the expression levels of AFP and GPC3 tumor markers. The Real time PCR analysis showed that lectin treated cells had a significant decrease of 90% and 89% in transcription levels of AFP and GPC3 respectively at a concentration of 175 μg/mL, thus suggesting strong anti-cancerous potential of Chenopodium album derived lectin. Lectins from plant sources induce apoptosis and autophagy of cancer cells and hence possess the potential of being developed as anti-cancer CaLec drug.

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Funding

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Authors and Affiliations

  1. Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan

    Mahwish Javed & Arif Malik

  2. Centre of Excellence in Molecular Biology, University of the Punjab Lahore, Lahore, Pakistan

    Muhammad Bilal, Bushra Tabassum, Olawale Samuel Adeyinka, Muhammad Tariq & Idrees Ahmad Nasir

  3. School of Biological Sciences, University of the Punjab Lahore, Lahore, Pakistan

    Bushra Tabassum

  4. Department of Biological Sciences, Afe Babalola University, Ado-Ekiti, Ekiti State, Nigeria

    Olawale Samuel Adeyinka

Authors
  1. Mahwish Javed
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  2. Muhammad Bilal
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  3. Bushra Tabassum
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  4. Arif Malik
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  5. Olawale Samuel Adeyinka
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  6. Muhammad Tariq
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  7. Idrees Ahmad Nasir
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Contributions

MJ Investigation. MB Investigation. BT Writing- Reviewing and Editing, data curation. AM Supervision. OSA Resources. MT real time qPCR assays. IAN Supervision and conceptulization.

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Correspondence to Bushra Tabassum.

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The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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Javed, M., Bilal, M., Tabassum, B. et al. Purification and functional characterization of lectin from Chenopodium album. J Proteins Proteom 13, 55–62 (2022). https://doi.org/10.1007/s42485-022-00084-3

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  • DOI: https://doi.org/10.1007/s42485-022-00084-3

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