Abstract
Seabuckthorn (Hippophae), is a stress hardy Himalayan shrub adapted to extreme climatic conditions. Still, very little is known about their stress tolerance mechanism. Therefore, aim of the present investigation is to understand the diverse stress hardiness mechanism of Indian seabuckthorn, growing naturally in high-altitude ‘Trans-Himalayan’ (H. rhamnoides, H. tibetana from Lahaul-Spiti, Himachal Pradesh) and lower altitude adapted ‘Sikkim’ (H. salicifolia) populations. Proteome data set generated using the comparative shotgun (nano LC-MS/MS) proteomics approach is the first protein repository of naturally growing Indian seabuckthorn populations. Bioinformatics analysis (KEGG and GO) revealed higher number of proteins associated with cellular, metabolic and biological regulation of physiological processes in Sikkim populations. Conversely, the abundance of proteins associated with redox signaling and response to stimulus indicates better stress-adaptive responses in higher altitude adapted Trans-Himalayan populations to withstand harsh climatic conditions. Interestingly, a positive correlation between proteomic plasticity and corresponding morphological (retarded growth, waxy/silvery-greyish leaf) and biochemical signatures (higher flavonoid and PUFA content) also supported efficient stress adaptive responses in Trans-Himalayan populations to survive harsher alpine climatic conditions. On the contrary, in Sikkim populations ‘H. salicifolia’ proteomic and biochemical (higher phenolics) resilience reflected the underlying physiological adaptive mechanisms to survive sub-alpine climatic conditions at lower elevations. To sum up, these interesting findings showed a clear trade-off between growth and stress tolerance phenomenon in diverse seabuckthorn populations. Trans-Himalayan populations repress their growth and divert energy resources in the direction of better stress responses to survive extreme climatic conditions. In contrast, Sikkim populations at lower elevations may invest in resource allocation or growth-promoting pathways in response to milder stress conditions. To the best of our knowledge, this is the first comprehensive proteome analysis to examine stress acclimation strategies in different naturally growing Indian seabuckthorn populations. The dataset is submitted in PRIDE ‘PRoteomics IDEntifications database’ proteomics data repository with accession number “PXD023184”.
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Acknowledgements
This work was supported by financial assistance from Department of Biotechnology (IBSD/A1/P(PH-2)/4) to RD. BS is thankful to DBT for providing fellowship.
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42485_2021_63_MOESM1_ESM.xlsx
Supplementary file1 Dataset containing complete list of common and differentially accumulated proteins in Trans-Himalayan and Sikkim seabuckthorn leaves (XLSX 29 kb)
42485_2021_63_MOESM2_ESM.xlsx
Supplementary file2 List of unique proteins identified in leaves of H.rhamnoides, H.tibetana and H.salicifolia (XLSX 29 kb)
42485_2021_63_MOESM4_ESM.xlsx
Supplementary file4 Comparative proteome profiling in Trans-Himalayan and Sikkim Himalayan seabuckthorn leaves hints towards differential stress adaptive responses (DOCX 219 kb)
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Sharma, B., Deswal, R. “Comparative proteome profiling of seabuckthorn leaves from low altitude ‘Sikkim’ and high altitude ‘Himachal Pradesh’ Himalayan region hints towards differential stress adaptive responses”. J Proteins Proteom 12, 125–141 (2021). https://doi.org/10.1007/s42485-021-00063-0
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DOI: https://doi.org/10.1007/s42485-021-00063-0