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Identification and validation of blast resistance Pi1 gene in the derived mapping population of rice variety, NLR34449 through molecular markers

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Abstract

NLR34449 is a popular, blast resistant, high-yielding variety, with highly desired fine-grain type and hence commands a premium price in the market. The nature and genetics of resistance to blast disease in this variety was not reported previously. Molecular marker-based screening using gene linked markers for ten blast resistance genes showed amplification of the resistance allele only with respect to the marker RM224, which is specific for Pi1 gene. In order to confirm the role of Pi1 gene with respect to blast disease resistance in Nellore Mahsuri, a F2 mapping population derived from the cross Nellore Mahsuri/Improved Samba Mahsuri (blast susceptible variety) was studied. The F2 plants segregated in ratio of 3 resistant: 1 susceptible indicating monogenic, dominant nature of inheritance with respect to blast resistance. Thus, the current study demonstrated that blast resistance in NLR34449 is contributed by Pi1.

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Acknowledgements

The authors would like to acknowledge the funding support provided by the Bayer Company for necessary support in doing research. The authors would also like to thank Director, ICAR-IIRR and ANGRAU, LAM, Guntur for providing all the necessary facilities to carry out the research work

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Authors and Affiliations

  1. ICAR-Indian Institute of Rice Research (ICAR-IIRR), Rajendranagar, Hyderabad, 500030, India

    Dasari Aleena, G. Rekha, M. S. Prasad, M. S. Madhav, E. Punniakoti, T. Dilip, P. Sinha, M. Kousik, M. A. Das, M. Anila, Divya Balakrishnan & R. M. Sundaram

  2. Acharya N.G. Ranga Agricultural University (ANGRAU), Lam, Guntur, India

    Dasari Aleena, V. Padma, Lal Ahmmed, D. Ratna Babu, A. Vijay Gopal & J. V. Ramana

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  1. Dasari Aleena
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  2. V. Padma
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  3. G. Rekha
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  4. M. S. Prasad
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  5. M. S. Madhav
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  6. E. Punniakoti
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  7. T. Dilip
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  8. P. Sinha
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  9. M. Kousik
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  10. M. A. Das
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  11. M. Anila
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  12. Lal Ahmmed
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  15. J. V. Ramana
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  16. Divya Balakrishnan
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  17. R. M. Sundaram
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Contributions

RMS conceptualised the experiment and supervised and suggested the key notes for the development of the draft. AD carried out the lab experiments as per the designed protocol and drafted the manuscript; VP provided the material required for the experiment and provided necessary inputs in the manuscript. MSM provided the required primers for carrying out the experiment. GR helped in carrying out the experiment in the lab, DB edited and improved the manuscript. All authors contributed in proof reading the manuscript and its improvement.

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Correspondence to Dasari Aleena or R. M. Sundaram.

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Aleena, D., Padma, V., Rekha, G. et al. Identification and validation of blast resistance Pi1 gene in the derived mapping population of rice variety, NLR34449 through molecular markers. Indian Phytopathology 76, 1091–1097 (2023). https://doi.org/10.1007/s42360-023-00688-9

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