Abstract
A set of 41 fluorescent pseudomonads isolates was isolated from 290 rhizosphere soil samples collected from six North East states of India with different agroclimatic zones. Differentiations of fluorescent Pseudomonas into different groups was made based on phenotypic characters. Among a subset of 28 isolates, 21 were grouped as Pseudomonas fluorescens Biovar I, 3 as Biovar II, 3 as Biovar III and 1 as Biovar V. The isolates Pf 8, Pf4 and Pf 39 belonging to Biovar I and II showed highest antagonistic potential against Ralstonia solanacearum with 86%, 68% and 64% growth suppression respectively in in vitro modified filter paper disc assay. Further, BLAST analyses of 16S rDNA sequences of representative seven isolates from six states revealed 94–99% similarity with Pseudomonas sp. In addition, three best isolates were used to prepare three substrate based bioformulation to perform an in Planta study. The in Planta study revealed among the tested bioformulations, Pf39-based bioformulation exhibited lowest wilt incidence in tomato and chilli plants. A negative correlation between population densities of R. solanacearum and Pseudomonas spp. as well as between Pseudomonas and percent wilt incidence was observed. Based on these observations Pf39 was found to be the most effective biocontrol agent against R. solanacearum under the experimental conditions.
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The 16S rDNA sequences of the Pseudomonas isolates were submitted to GeneBank database (https://www.ncbi.nlm.nih.gov/genbank/) and the accession numbers obtained are given in the Supplementary file (Table S6).
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The first author received Moulana Azad National Fellowship for Minority Students under University Grant Commission (UGC), India. The authors are grateful to the anonymous reviewers for their valuable suggestions that help improve the manuscript.
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TLK and LCB: conceived the idea; TLK, LCB, PKB, MB, KCP: study design, supervision of experiments and revising the draft manuscript; TLK: sample collection, Isolation and screening of the isolates, pot experiment; GG and TLK: molecular characterization, writing the draft manuscript. All the authors have read the manuscript and agreed to submit in this journal.
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Kipgen, T.L., Bora, L.C., Goswami, G. et al. Isolation and characterization of fluorescent Pseudomonas with bio-control potential against Ralstonia solanacearum. Indian Phytopathology 74, 1055–1064 (2021). https://doi.org/10.1007/s42360-021-00400-9
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DOI: https://doi.org/10.1007/s42360-021-00400-9