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Effect of pure culture and culture filtrates of Trichoderma species on root-knot nematode, Meloidogyne incognita infesting tomato

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Abstract

Studies were carried out to evaluate the effectiveness of soil application of culture suspension and culture filtrate of five Trichoderma spp. viz., T. harzianum, T. hamatum, T. viride, T. virens and T. koningii against root-knot nematode, Meloidogyne incognita (J2) on tomato cv. K-21. The host cultivar was found highly susceptible to the nematode and developed 117 characteristic oval and fleshy galls and 92 egg masses/root system at an inoculum level of 2000 J2/kg soil. The nematode infection caused 18–23% decrease in the plant growth and yield (16–30%) variables of tomato. Soil application of Trichoderma spp. suppressed the galling, egg mass production, fecundity and soil population of the nematode and improved the plant growth of tomato that varied with treatment. The culture suspension of T. harzianum provided maximum control (30%) of the nematode and increased the plant growth (19–22%), biomass production (16–20%) and yield (29%) of tomato over uninoculated control followed by yield enhancement with T. hamatum (13–16%) and T. virens (9–13%). All tested Trichoderma species established in soil and their populations increased over time; however, nematode population decreased with the progress of time. Among the tested Trichoderma spp., T. harzianum performed better against the M. incognita. The treatments with culture filtrate also suppressed the root-knot and improved the tomato yield, but it was found 6–11% less effective than the culture suspension.

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Authors and Affiliations

  1. Department of Plant Protection, Faculty of Agriculture Science, Aligarh Muslim University, Aligarh, 202 002, Uttar Pradesh, India

    Mujeebur Rahman Khan, Irfan Ahmad & Faheem Ahamad

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  1. Mujeebur Rahman Khan
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  2. Irfan Ahmad
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  3. Faheem Ahamad
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Correspondence to Mujeebur Rahman Khan.

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Khan, M.R., Ahmad, I. & Ahamad, F. Effect of pure culture and culture filtrates of Trichoderma species on root-knot nematode, Meloidogyne incognita infesting tomato. Indian Phytopathology 71, 265–274 (2018). https://doi.org/10.1007/s42360-018-0031-1

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  • DOI: https://doi.org/10.1007/s42360-018-0031-1

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