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Grapevine cultivar Shine Muscat in China: occurrence of viruses and attempts to produce certified propagation material

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Abstract

Virus diseases in grapevine ‘Shine Muscat’, an economically significant table grape cultivar in China, have become a major concern for grapevine growers. To investigate which viruses are frequent in ‘Shine Muscat’ and screen for healthy propagation material for this cultivar, a total of 140 samples (dormant canes) of ‘Shine Muscat’ plants were collected from 45 vineyards in different viticultural areas in China. RT-PCR was conducted to detect the presence of grapevine leafroll-associated virus 1 (GLRaV-1), GLRaV-2, GLRaV-3, grapevine fanleaf virus (GFLV), grapevine fleck virus (GFkV), grapevine virus A (GVA), grapevine rupestris stem pitting-associated virus (GRSPaV), grapevine Pinot gris virus (GPGV) and grapevine berry inner necrosis virus (GINV). No virus-free cane was found and all samples were infected with a mixture of at least two viruses; double, triple and quadruple infections accounted for about 31%, 30%, and 22% of the infections, respectively. The most frequent viruses in ‘Shine Muscat’ plants were GPGV (67.86%), GVA (59.29%) and GRSPaV (55.0%), and GFLV and GLRaV-1 were not detected in any of the samples. In vitro-grown ‘Shine Muscat’ plantlets carrying GFkV/GINV/GVA/GRSPaV were subjected to thermotherapy or chemotherapy followed by shoot-tip culture. GINV, GFkV and GVA were easily eliminated from the cultivar by both sanitation methods, whereas the frequency of GRSPaV elimination was relatively low. The results presented here contribute to our understanding of virus diseases in grapevine cv. Shine Muscat and to improving the sanitary status of the cultivar’s planting material.

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Data Availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgements

We thank for Mr. Xuechi Fu (China Agricultural University) for his help in sampling. This research was supported by National Natural Science Foundation of China (No. 32072552), the Earmarked Fund for Modern Agro-Industry Technology Research System (CARS-29-bc-3) and the Construction of Beijing Science and Technology Innovation and Service Capacity in Top Subjects (CEFF-PXM2019_014207_000032).

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Author notes

  1. Shangzhen Yu and Qing Kan contributed equally to this work.

Authors and Affiliations

  1. Department of Pomology/Lab of Stress Physiology and Molecular Biology for Tree Fruits, a Key Laboratory of Beijing Municipality, China Agricultural University, Beijing, 100193, China

    Shangzhen Yu, Qing Kan, Haoqiang Huang, Jinying Wang, Yinshuai Xie, Hanwei Li, Xueqing Zhang, Can Liu & Yuqin Cheng

  2. Key Laboratory of Viticulture and Enology, Ministry of Agriculture, Beijing, 100083, China

    Yuqin Cheng

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  1. Shangzhen Yu
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  2. Qing Kan
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Contributions

YC, SY, and QK conceived the study and designed the research. SY, QK, HH, JW and YX performed the sampling. SY and HH conducted the virus detection. QK and HH conducted the virus elimination. JW, YX and HL performed sample handling and data analyses. XZ and CL established the in vitro cultures. YC wrote the manuscript and discussed the results, and all of the authors commented on the manuscript.

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Correspondence to Yuqin Cheng.

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Yu, S., Kan, Q., Huang, H. et al. Grapevine cultivar Shine Muscat in China: occurrence of viruses and attempts to produce certified propagation material. J Plant Pathol 105, 1609–1616 (2023). https://doi.org/10.1007/s42161-023-01508-9

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  • DOI: https://doi.org/10.1007/s42161-023-01508-9

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