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Evidence for seed transmission of sweet potato symptomless virus 1 in sweet potato (Ipomoea batatas)

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Abstract

Sweet potato symptomless virus 1 (SPSMV-1) is a member of genus Mastrevirus, in the family Geminiviridae. SPSMV-1 was detected from sweet potato (Ipomoea batatas) seedlings by small-RNA deep-sequencing. PCR analysis indicated that SPSMV-1 was detected in 12 out of 114 individual sweet potato seedlings from seeds collected from a SPSMV-1-infected sweet potato plant, and additionally, detected in whole seeds, seed coats attached with endosperm, and in embryos. The complete genome of the virus was subsequently cloned by using a polymerase chain reaction (PCR) method with back-to-back (full) primer pairs from sweet potato seedlings and seeds. In summary, the results suggest that SPSMV-1 may be a seed-borne virus and a seed transmissible virus in sweet potato. To our knowledge, this is the first evidence for seed transmission of SPSMV-1, and seed transmission under natural conditions for any mastrevirus as well.

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Acknowledgments

This work was financially supported by a grant from the Earmarked Fund for China Agriculture Research System (CARS-10-B13) and a project from Industry-University-Research Cooperation of Henan Province of China (172107000038). We are thankful to Dr. Li DaWei from China Agricultural University and Dr. Cao MengJi from Southwest University of China for conducting partial sequence analysis of small RNAs and critically reading the manuscript.

Funding

This study was funded by Earmarked Fund for China Agriculture Research System (CARS-10-B13) and Industry-University-Research Cooperation of Henan Province of China (172107000038).

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Authors and Affiliations

  1. Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China

    Qi Qiao, ZhenChen Zhang, XiaoLi Zhao, YongJiang Wang, Shuang Wang, YanHong Qin, DeSheng Zhang, YuTing Tian & Fumei Zhao

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  1. Qi Qiao
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  2. ZhenChen Zhang
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  3. XiaoLi Zhao
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  7. DeSheng Zhang
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  8. YuTing Tian
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  9. Fumei Zhao
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Correspondence to ZhenChen Zhang.

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Dr. Qi Qiao declares that he has no conflict of interest. Dr. ZhenChen Zhang declares that he has no conflict of interest. Miss XiaoLi Zhao declares that she has no conflict of interest. Dr. YongJiang Wang declares that he has no conflict of interest. Dr. Shuang Wang declares that she has no conflict of interest. Dr. YanHong Qin declares that she has no conflict of interest. Mr. DeSheng Zhang declares that he has no conflict of interest. Miss YuTing Tian declares that she has no conflict of interest. Dr. Fumei Zhao declares that she has no conflict of interest.

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Qiao, Q., Zhang, Z., Zhao, X. et al. Evidence for seed transmission of sweet potato symptomless virus 1 in sweet potato (Ipomoea batatas). J Plant Pathol 102, 299–303 (2020). https://doi.org/10.1007/s42161-019-00427-y

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  • DOI: https://doi.org/10.1007/s42161-019-00427-y

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