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Behavioural responses of bone-like cells on dense and porous dicalcium phosphate dihydrate-coated β-tricalcium phosphate granules

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Abstract

The present study investigated the behavioural response of bone-like cells towards dense and porous dicalcium phosphate dihydrate (DCPD)-coated β-tricalcium phosphate (β-TCP) granules. The surfaces of the dense and porous β-TCP granules were coated with a layer of DCPD through the dissolution-precipitation process by exposing them to an acidic calcium phosphate solution for 30 min at 25 °C. Subsequently, the specimens were characterised with X-ray diffraction (XRD), scanning electron microscope (SEM), dissolution and pH tests, and evaluation of initial cell attachment. The results demonstrated that the porous β-TCP granule surfaces coated with a DCPD layer contributed to the high concentration of calcium ions released and decreased pH values. Furthermore, the DCPD-coated porous β-TCP granules produced good initial cell attachment and enhanced cell viability for up to three days. The results would provide new insights to bioceramic researchers regarding the impacts of pore presence in accelerating DCPD-coated β-TCP granule cell responses.

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Acknowledgements

The corresponding author would like to thank the Malaysian Ministry of Higher Education for funding the study via Fundamental Research Grant Scheme (Project Code: FRGS/1/2020/TK0/USM/03/1).

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Authors and Affiliations

  1. School of Materials and Mineral Resources Engineering, Engineering Campus, Universiti Sains Malaysia, 14300, Nibong Tebal, Penang, Malaysia

    Nur Zulaikha Mohamad Zaidi, Ahmed Hafedh Mohammed Mohammed & Khairul Anuar Shariff

  2. Department of Dental Science, Advanced Medical and Dental Institute, Universiti Sains Malaysia, 13200, Kepala Batas, Malaysia

    Mamun Khan Sujon

  3. Bioprocess Technology Division, School of Industrial Technology, Universiti Sains Malaysia, 11800, Penang, Malaysia

    Mohamad Hafizi Abu Bakar

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  1. Nur Zulaikha Mohamad Zaidi
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Correspondence to Khairul Anuar Shariff.

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Mohamad Zaidi, N.Z., Mohammed, A.H.M., Sujon, M.K. et al. Behavioural responses of bone-like cells on dense and porous dicalcium phosphate dihydrate-coated β-tricalcium phosphate granules. J Aust Ceram Soc 59, 1423–1431 (2023). https://doi.org/10.1007/s41779-023-00925-4

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