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Fe2+/Fe3+ Conversation-Mediated Magnetic Relaxation Switching for Detecting Staphylococcus Aureus in Blood and Abscess via Liposome Assisted Amplification

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Abstract

Rapid and accurate detection of pathogenic bacteria of Staphylococcus aureus (S. aureus) is of importance in the field of clinical research and practical application. Herein, we report a magnetic relaxation switching (MRS)-based strategy for detecting S. aureus in complex samples via the assistance of signal amplification system of liposome. In this design, vancomycin (Van) modified 200 nm magnetic beads (MB200-Van) and biotinylated immunoglobulin (Biotin-IgG) were employed as capture probes for S. aureus which following labelled by biotinylated liposomes loading with glucose molecule (Biotin-liposome@Glu, BLGs) based on the affinity of biotin with streptavidin. The released glucose molecules combining with glucose oxidase generated Fe3+, causing transverse relaxation time (T2) signal change. The combination of dual recognition of S. aureus, liposome assisted signal amplification, and the MRS-based assay achieved direct and specific detection of S. aureus in blood and abscess samples. The as-proposed method for S. aureus assay in this study shows potential application for infectious diagnosis in clinical.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (22174116, 21974110), and Chongqing Science Funds for Distinguished Young Scientists (cstc2021jcyj-jqx0024), and the Innovation Research Group at higher Education Institutions in Chongqing, Chongqing Education Committee (CXQT21006).

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Authors and Affiliations

  1. Key Laboratory of Luminescence Analysis and Molecular Sensing, College of Pharmaceutical Sciences, Southwest University, Chongqing, 400715, China

    Xin Guo, Xin-Chun Deng, Ya-Qing Zhang, Qin Luo, Xiao-Kang Zhu & Er-Qun Song

  2. State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental Sciences, CAS, Beijing, 100085, China

    Yang Song

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  1. Xin Guo
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Correspondence to Er-Qun Song.

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Guo, X., Deng, XC., Zhang, YQ. et al. Fe2+/Fe3+ Conversation-Mediated Magnetic Relaxation Switching for Detecting Staphylococcus Aureus in Blood and Abscess via Liposome Assisted Amplification. J. Anal. Test. 6, 111–119 (2022). https://doi.org/10.1007/s41664-022-00227-x

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  • DOI: https://doi.org/10.1007/s41664-022-00227-x

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