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Elicitation Improves Phenolic Acid Content and Antioxidant Enzymes Activity in Salvia leriifolia Cell Cultures

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Iranian Journal of Science and Technology, Transactions A: Science Aims and scope Submit manuscript

Abstract

Elicitors, such as salicylic acid (SA), stand as the effective agents that are applied to plant cell suspension cultures for the fabrication of biomaterials. At this project, we have studied the elicitor effects on the increase in phenols, PAL activities, POX, and CAT enzymes that exist in the cell culture of Salvia leriifolia. Upon the addition of SA to the cell culture, an increase has been detected in the phenols (CA and Sal B) and the activity of PAL, POX, and CAT enzymes. The increases of phenols and PAL activity have been stimulated 48 h after being treated with SA and the activities of resulting antioxidant enzymes have been detected to be greatly improved. Elicitation of SA on phenols gathering has been dependent on the applied dose and time period. The appropriate SA assemblage for eliciting phenols accumulations has been determined to be 10 mg/L. Elicitation influence of SA on phenols gatherings has associated with the rate of PAL, indicating that PAL may be the crucial role for the synthesis of Sal B and CA. The increased PAL activity can lead to the progress of phenolic compounds amount, which is quite significant in regards to the usage of cell culture systems for biofabrication of secondary metabolites such as CA and Sal B.

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Abbreviations

SA:

Salicylic acid

PAL:

Phenyl Alanine Ammonialyase

POX:

Peroxidase

CAT:

Catalase

CA:

Caffeic Acid

Sal B:

Salvianolic acid B

NAA:

Naftalen Acetic Acid

BAP:

Benzil Amino Purin

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Acknowledgements

The authors thank the Farhangian University of Mashhad for providing the required equipments and facilities.

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, Farhangian University of Khorasan Razavi, Mashhad, Iran

    Masoomeh Modarres

  2. Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Mohammad Ehsan Taghavizadeh Yazdi

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  1. Masoomeh Modarres
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  2. Mohammad Ehsan Taghavizadeh Yazdi
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Correspondence to Masoomeh Modarres or Mohammad Ehsan Taghavizadeh Yazdi.

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Modarres, M., Taghavizadeh Yazdi, M.E. Elicitation Improves Phenolic Acid Content and Antioxidant Enzymes Activity in Salvia leriifolia Cell Cultures. Iran J Sci Technol Trans Sci 45, 849–855 (2021). https://doi.org/10.1007/s40995-021-01070-y

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