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Production of Phytotoxic Metabolites with Bioherbicidal Activities from Lasiodiplodia pseudotheobromae Produced on Different Agricultural Wastes Using Solid-State Fermentation

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Abstract

This study was carried out to optimize and enhance the production of phytotoxic metabolites from Lasiodiplodia pseudotheobromae (C1136) for its herbicidal properties using solid-state fermentation with various agricultural wastes available in Nigeria. The results obtained showed that banana peel (10.12 ± 0.10 g/100 g) and wheat bran (9.89 ± 0.11 g/100 g) had the highest protein content. Banana peel was observed to have the highest value of phosphorus (0.30 ± 0.05 g/100 g), closely followed by wheat bran (0.23 ± 0.06 g/100 g). Higher values of arginine contents were obtained for rice bran (3.06 ± 0.06 g/100 g), groundnut wastes (2.98 ± 0.08 g/100 g) and wheat bran (2.38 ± 0.08 g/100 g). These values are significant (p < 0.05). Endoamylase (64 U/g), exoamylase (50 U/g), isoamylase (43 U/g), protease (15 U/g), xylanase (12 U/g), cellulose (4.8 U/g) enzymes that were obtained from groundnut waste showed the best activities when compared to other substrates. It was observed that as the concentration of the extract increases so also are the radicle length, shoot length and germination rate on the tested seeds. Leaf necrosis assay showed that the phytotoxic metabolite from strain C1136 produced the largest necrotic area (3.2 ± 0.2 mm) on Amaranthus hybridus leaves when grown on groundnut waste. In comparison, the necrotic area of (2.5 ± 0.2 mm) was observed for Echinochloa crus-galli leave from phytotoxic metabolites grown on pineapple waste. The efficacy of the phytotoxic metabolites produced from strain C1136 in this study potends a strong solution for the environmental and human health challenges alludes to chemical herbicides.

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Authors and Affiliations

  1. Applied Microbiology, Biotechnology and Nanotechnology Laboratory, Department of Microbiology, Landmark University, Omu-Aran, Nigeria

    Charles Oluwaseun Adetunji & Oghenerobor Benjamin Akpor

  2. Department of Animal Science, Animal Nutrition, Biotechnology and Food Safety Laboratory, Federal University, Gashua, Nigeria

    Isaac Oluseun Adejumo

  3. Department of Pure and Applied Biology, Ladoke Akintola University of Technology, P.M.B 4000, Ogbomosho, Oyo State, Nigeria

    Julius Kola Oloke

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  1. Charles Oluwaseun Adetunji
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  2. Isaac Oluseun Adejumo
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  3. Julius Kola Oloke
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  4. Oghenerobor Benjamin Akpor
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Correspondence to Charles Oluwaseun Adetunji.

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Adetunji, C.O., Adejumo, I.O., Oloke, J.K. et al. Production of Phytotoxic Metabolites with Bioherbicidal Activities from Lasiodiplodia pseudotheobromae Produced on Different Agricultural Wastes Using Solid-State Fermentation. Iran J Sci Technol Trans Sci 42, 1163–1175 (2018). https://doi.org/10.1007/s40995-017-0369-8

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