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Decellularization and Recellularization of Rabbit Kidney Using Adipose-Derived Mesenchymal Stem Cells for Renal Tissue Engineering

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A Correction to this article was published on 14 June 2021

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Abstract

Purpose

The effectiveness of decellularization and recellularization of rabbit kidney scaffolds with autologous adipose-derived mesenchymal stem cells (ADMSCs) was assessed. The static-based technique was judged against the perfusion-based method both in decellularization and recellularization processes.

Methods

Kidneys were obtained from 8 rabbits and divided into two groups. Two kidneys did not undergo any processing and were served as the control group. Kidneys of group I (N = 7) were cannulated via the renal artery and decellularized with a detergent-based method. We reseeded these constructs with ADMSCs using a peristaltic pump. The static method was applied to decellularize and recellularize thin slices of the seven kidneys (group II, N = 7) using the L929 cell line. Several tests were performed to evaluate the efficacy of the decellularization and recellularization processes.

Results

All cellular and nuclear constituents were eliminated from the scaffolds of both groups confirmed with hematoxylin and eosin (H&E), trichrome, 4′,6-diamidino-2-phenylindole (DAPI) staining, and DNA quantification. Cell viability was over 89% as performed by MTT assay. Cells were effectively seeded in scaffolds of both groups, with a superior arrangement and a higher concentration in the perfusion-based technique. Immunohistochemistry (IHC) staining demonstrated preservation of native expression patterns of extracellular matrix proteins with better results in perfusion-based technique.

Conclusion

The results suggest that organs with the distributed micro-vasculature system (perfusion method) may benefit from being engineered with the dynamic procedure compared with organs with empty chambers and poor vasculature system (static system).

Lay Summary

Previously, we evaluated the efficacy of the transplantation of decellularized kidneys in a rat model. In this study, we try to assess the efficacy of two decellularization and recellularization protocols of kidney organs for further renal tissue engineering. We also aim to produce renal scaffolds with structural, mechanical, and physiological characteristics that are crucial for engineering main renal structures, using adipose-derived mesenchymal stem cells.

Future Studies

Finding the most suitable and feasible decellularization and recellularization methods is important in several medicinal therapeutics. In the future, we will emphasize on the long-term viability and functionality of obtained kidney scaffolds in clinically translatable applications. We will also compare different cell lineages to obtain the most efficient scaffold for further renal tissue engineering.

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Funding

The authors thank the Tehran University of Medical Sciences for funding this study (Grant Number 32213).

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Authors and Affiliations

  1. Pediatric Urology and Regenerative Medicine Research Center, Section of Tissue Engineering and Stem Cells Therapy, Tehran, Iran

    Shabnam Sabetkish, Nastaran Sabetkish, Masoumeh Ekhtiari, Bahareh Mohammadi Jobani & Abdol-Mohammad Kajbafzadeh

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  1. Shabnam Sabetkish
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  2. Nastaran Sabetkish
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  3. Masoumeh Ekhtiari
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  4. Bahareh Mohammadi Jobani
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  5. Abdol-Mohammad Kajbafzadeh
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Correspondence to Abdol-Mohammad Kajbafzadeh.

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The authors declare that they have no conflict of interest.

Ethical Approval

All procedures performed in this study were in accordance with the ethical standards and under guidelines approved by the local ethical committee of the Tehran University of Medical Sciences. The animal experiment complies with the National Institutes of Health guide for the care and use of laboratory animals (NIH Publications No. 8023, revised 1978).

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Sabetkish, S., Sabetkish, N., Ekhtiari, M. et al. Decellularization and Recellularization of Rabbit Kidney Using Adipose-Derived Mesenchymal Stem Cells for Renal Tissue Engineering. Regen. Eng. Transl. Med. 6, 433–441 (2020). https://doi.org/10.1007/s40883-020-00177-9

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  • DOI: https://doi.org/10.1007/s40883-020-00177-9

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