Abstract
To understand the regulation patterns of microRNAs (miRNAs) under salt stress in Tartary buckwheat, we performed miRNAs sequencing analysis under normal and salt condition (100 mM NaCl), which provided a theoretical basis for the research on specific genes in Fagopyrum tataricum. Two varieties of Chuanqiao No.1 (WT1) and Chuanqiao No.2 (WT2), and two mutants of Chuanqiao No.1-1 (M1) and Chuanqiao No.2-1 (M2) (screened from saline-alkali soil after WT1 and WT2 natural mutation) of Tartary buckwheat were used as plant materials. Such plant materials, exhibiting three leaves, were subjected to 100 mM NaCl for two days. A total of 770 known miRNAs and 264 novel miRNAs were measured in different Tartary buckwheat cultivars, and the expression of 19 miRNAs was affected by salt. Meanwhile, 10 target genes were cleaved by the corresponding miRNAs, such as miR156-z, miR157-x, miR172-x and miR393-y, and are predicted to be associated with metabolic processes, cellular process, and biological regulation. Nearly half of the target genes are classified as transcription factors (TFs), like CO-LIKE, SBP, AP2 and NF-X1. Differential expression analysis revealed that 31 miRNAs and 35 miRNAs were differentially expressed in the combination of WT1-CK vs WT1-T and M1-CK vs M1-T, respectively, whereas 13 miRNAs and 60 miRNAs were differentially expressed in the combination of WT2-CK vs WT2-T and M2-CK vs M2-T, respectively. Here we show evidence of the regulation and roles of miRNAs and mRNA in Tartary buckwheat as it may provide a new molecular basis for further studies on salt tolerance mechanisms.
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Acknowledgements
We would like to acknowledge financial support from the Natural Science Foundation of Shandong Province (ZR2020MC086) and the National Natural Science Foundation of China (31371552), and acknowledge Dr. Mark Ziemann of Deakin University in Australia for language editing. The research materials Chuanqiao No.1 and Chuanqiao No.2 were furnished by the Research Station of Alpine Crop, Department of Agriculture and Science in Liangshan State, Sichuan Province, China.
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SJN analyzed the transcriptome data and wrote the manuscript. WYQ analyzed the salt tolerance of four Tartary buckwheat cultivars. YHB designed the experiment and revised the manuscript.
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40626_2022_262_MOESM3_ESM.xls
Supplementary Table S3 Different expression pattern of miRNAs. A: WT1-CK/WT1-T, B: M1-CK/M1-T, C: WT2-CK/WT2-T, D: M2-CK/M2-T (XLS 50 kb)
40626_2022_262_MOESM5_ESM.xls
Supplementary Table S5 Gene ontology of DEGs. A: WT1-CK/WT1-T, B: M1-CK/M1-T, C: WT2-CK/WT2-T, D: M2-CK/M2-T (XLS 81 kb)
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Song, JN., Wang, YQ. & Yang, HB. MicroRNA sequencing reveals the salt responses in four Tartary buckwheat cultivars. Theor. Exp. Plant Physiol. 34, 517–536 (2022). https://doi.org/10.1007/s40626-022-00262-0
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DOI: https://doi.org/10.1007/s40626-022-00262-0