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Can the positive association of osteocalcin with testosterone be unmasked when the preeminent hypothalamic–pituitary regulation of testosterone production is impaired? The model of spinal cord injury

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Abstract

Purpose

Osteocalcin (OCN), released from the bone matrix during the resorption phase, in its undercarboxylated form, stimulates testosterone (T) biosynthesis in mouse and a loss-of-function mutation of its receptor was associated with hypergonadotropic hypogonadism in humans. Nevertheless, when population-based studies have explored the OCN-T association, conflicting results have been reported. Hypothesizing that the evidence of a positive association between OCN and T could have been hindered by the preeminent role of a well-functioning hypothalamus–pituitary axis in promoting T biosynthesis, we explored this association in men with chronic spinal cord injury (SCI), exhibiting high prevalence of non-hypergonadotropic androgen deficiency.

Methods

Fifty-five consecutive men with chronic SCI underwent clinical/biochemical evaluations, including measurements of total T (TT), OCN and 25(OH)D levels. Free T (FT) levels were calculated by the Vermeulen formula. Comorbidity was scored by Charlson comorbidity index (CCI).

Results

A biochemical androgen deficiency (TT < 300 ng/dL) was observed in 15 patients (27.3%). TT was positively correlated with OCN, 25(OH)D and leisure time physical activity and negatively correlated with age, BMI and CCI. OCN was also positively correlated with calculated FT and negatively correlated with BMI and HOMA-IR. At the multiple linear regression analyses, a positive association of OCN with TT and calculated FT persisted after adjustment for confounders.

Conclusions

The positive association here found between OCN and T levels in men with chronic SCI reinforces the notion that a bone–testis axis is also functioning in humans and suggests that it can be unmasked when the preeminent hypothalamic–pituitary regulation of T production is impaired.

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Authors and Affiliations

  1. Spinal Unit, San Raffaele Sulmona Institute, 67039, Sulmona, Italy

    A. Barbonetti & G. Felzani

  2. Andrology Unit, Department of Life, Health and Environment Sciences, University of L’Aquila, 67100, L’Aquila, Italy

    S. D’Andrea, A. Martorella, S. Francavilla & F. Francavilla

  3. Endocrinology Unit, Department of Experimental and Clinical Biomedical Sciences, University of Florence, 50139, Florence, Italy

    J. Samavat & M. Luconi

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  1. A. Barbonetti
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Correspondence to A. Barbonetti.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Barbonetti, A., D’Andrea, S., Samavat, J. et al. Can the positive association of osteocalcin with testosterone be unmasked when the preeminent hypothalamic–pituitary regulation of testosterone production is impaired? The model of spinal cord injury. J Endocrinol Invest 42, 167–173 (2019). https://doi.org/10.1007/s40618-018-0897-x

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