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Single-Cell Transcriptome Analysis of Neural Stem Cells

  • Bioinformatics and Stem Cell (R Hart, Section Editor)
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Abstract

Neural stem cells (NSCs) in the adult central nervous system play essential roles in both normal homeostasis and repair of damaged tissue after injury. The study of adult NSCs is hampered by the heterogeneous NSC population. In this review, we describe recent progresses in using single-cell RNA-sequencing (scRNA-seq) technique for the investigation of NSCs. The first part of this review focuses on the scRNA-seq techniques and bioinformatic analysis. The second part emphasizes the applications of scRNA-seq analysis in NSC research. Finally, we discuss the challenges and future directions of scRNA-seq technique for both basic research and regenerative medicine.

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Acknowledgments

The work was supported in part by grants from the New Jersey Commission on Spinal Cord Research and Busch Biomedical Research.

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Authors and Affiliations

  1. Department of Biomedical Engineering, Rutgers University, 599 Taylor Road, Piscataway, NJ, 08854, USA

    Ying Li, Jeremy Anderson & Li Cai

  2. Department of Cell Biology & Neuroscience, Keck Center for Collaborative Research and Stem Cell Research Center, Rutgers University, 604 Allison Rd, Piscataway, NJ, 08854, USA

    Kelvin Y. Kwan

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  1. Ying Li
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  2. Jeremy Anderson
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Correspondence to Li Cai.

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Li, Y., Anderson, J., Kwan, K.Y. et al. Single-Cell Transcriptome Analysis of Neural Stem Cells. Curr Pharmacol Rep 3, 68–76 (2017). https://doi.org/10.1007/s40495-017-0084-3

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