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Atomically-precise Au24Ag1 Clusterzymes with Enhanced Peroxidase-like Activity for Bioanalysis

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Abstract

Atomically-precise clusterzymes have been widely studied for their special physicochemical properties, but it is still a challenge to enhance their peroxidase-like activity. Herein, we demonstrated that by substituting a single Ag atom into Au25 nanoclusters to form Au24Ag1 nanoclusters, the peroxidase-like activity was enhanced greatly. In the presence of H2O2, Au24Ag1 could produce reactive oxygen species (ROS) to oxidize colorless 3,3’5,5’-tetramethylbenzidine (TMB) to the blue oxidized TMB (oxTMB). It is worth mentioning that pyrophosphate compounds inhibit the activity of Au24Ag1. Since alkaline phosphatase (ALP) can dephosphorylate the substrate phosphate compound, that is, remove the phosphate group on the substrate by hydrolysis, the enzymatic activity of the clusterzyme is restored. Based on this, we have developed a sensitive and reliable colorimetric sensing system for the detection of pyrophosphate ion (PPi), adenosine triphosphate (ATP), adenosine diphosphate (ADP) and ALP, respectively. Importantly, the detection limit of the assay system is lower than those of most of the assays that have been reported. In addition, we also built a simple optical logic gate on this basis, further extending the application of metal nanoclusters as peroxidase mimics in bioanalysis. This work could help to shed light on the structure-activity relationship of nanozyme.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Nos.22172063 and 32202145), the Fund of Young Taishan Scholar Program, China(No.tsqn201812080), the Natural Science Foundation of Shandong Province, China(No.ZR2019YQ10) and the Independent Cultivation Program of Innovation Team of Ji’nan City, China(No.2021GXRC052).

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Authors and Affiliations

  1. School of Materials Science and Engineering, University of Jinan, Jinan, 250022, P. R. China

    Shuna Wang, Zhe Li, Mingyuan Xia, Xiaoxue Zhao, Chuanxia Chen, Yuanyuan Jiang, Pengjuan Ni & Yizhong Lu

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  1. Shuna Wang
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  2. Zhe Li
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  3. Mingyuan Xia
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  4. Xiaoxue Zhao
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  5. Chuanxia Chen
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  6. Yuanyuan Jiang
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  7. Pengjuan Ni
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  8. Yizhong Lu
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Correspondence to Yizhong Lu.

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Wang, S., Li, Z., Xia, M. et al. Atomically-precise Au24Ag1 Clusterzymes with Enhanced Peroxidase-like Activity for Bioanalysis. Chem. Res. Chin. Univ. 39, 907–914 (2023). https://doi.org/10.1007/s40242-022-2259-7

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  • DOI: https://doi.org/10.1007/s40242-022-2259-7

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