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Protective effect of cinnamon on diabetic cardiomyopathy in nicotinamide-streptozotocin induced diabetic rat model

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Abstract

There is an increase in the incidence and prevalence of type-2 diabetes and obesity which leads to the structural and functional changes in myocardium leading to a lethal complication called diabetic cardiomyopathy (DCM). In the present study, we investigated the preventive effect of cinnamon (3% of Cinnamomum zeylanicum bark powder in AIN-93 diet for 3 months) feeding on DCM and the concerned mechanisms in a rodent model. Experimental diabetes was induced by a single intraperitoneal injection of 40 mg/kg b.w streptozotocin (STZ), 15 min after the ip administration of 60 mg/kg b.w of nicotinamide (NA) in Wistar-NIN (WNIN) male rats. The oxidative stress parameters were investigated by assessing superoxide dismutase (SOD), glutathione-s-transferase (GST) enzyme activity, protein carbonyls and malondialdehyde (MDA) levels. The histopathology of myocardium was analyzed by H&E and Masson’s trichrome staining, and scanning electron microscopy. The changes in diabetic rat heart involved the altered left ventricular parietal pericardium, structural changes in myocardial cells, enhanced oxidative stress. Masson’s trichrome and H&E staining have shown increased fibrosis, and perinuclear vacuolization in NA-STZ induced diabetic rat myocardium. Cinnamon feeding prevented the oxidative stress and myocardial alterations in the heart of diabetic rats. Taken together, these results suggest that cinnamon can effectively prevent the metabolic and structural changes in NA-STZ induced diabetic cardiomyopathy.

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Abbreviations

T1D:

Type-1 diabetes

T2D:

Type-2 diabetes

DCM:

Diabetic cardiomyopathy

AGEs:

Advanced glycation end products

STZ:

Streptozotocin

NA:

Nicotinamide

VEGF:

Vascular endothelial growth factor

SOD:

Superoxide dismutase

GST:

Glutathione-s-transferase

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Acknowledgements

This work was supported by grants from Department of Biotechnology, Government of India to PS and GBR. CUK was supported by a research fellowship from the Indian Council of Medical Research, Government of India. We thank Khaja Ather Hussain for histological analysis and also Mr. Manohar Reddy for statistical analysis.

Funding

This work was supported by grants from Department of Biotechnology, Government of India to PS and GBR. CUK was supported by a research fellowship from the Indian Council of Medical Research, Government of India.

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Authors and Affiliations

  1. Biochemistry Division, ICMR- National Institute of Nutrition, Hyderabad, 500 007, India

    Chekkilla Uday Kumar, Singareddy Sreenivasa Reddy, Madhoosudan A. Patil, Periketi Madhusudana Chary & Geereddy Bhanuprakash Reddy

  2. Clinical Division, National Institute of Nutrition, Hyderabad, India

    Palla Suryanarayana

  3. Pathology Division, National Institute of Nutrition, Hyderabad, India

    Putcha Uday Kumar

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  1. Chekkilla Uday Kumar
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Correspondence to Geereddy Bhanuprakash Reddy.

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Kumar, C.U., Reddy, S.S., Suryanarayana, P. et al. Protective effect of cinnamon on diabetic cardiomyopathy in nicotinamide-streptozotocin induced diabetic rat model. J Diabetes Metab Disord 21, 141–150 (2022). https://doi.org/10.1007/s40200-021-00948-3

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