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Potential of Microrhizomes for In Vitro Gingerol and Shogaol Synthesis in Ginger (Zingiber officinale Rosc.)

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Five genotypes of ginger cultured in vitro produced calli and microrhizomes, and they were evaluated for pungency principles at different induction periods using HPLC. Differentiation and growth of microrhizomes and calli and yield of gingerols were found genotype dependent. Accumulation of oleoresin, gingerols and shogaol increased with time duration, reaching the highest after 3 months of induction. The microrhizomes and calli of genotype cv. Aswathy yielded the highest gingerols throughout the three-month induction period. However, microrhizomes accumulated 50 times (average 26.2 times) higher total gingerols compared to calli, irrespective of the genotypes. This study proved the potential of microrhizomes for in vitro gingerol synthesis. It is suggested that large-scale production of microrhizomes can partially substitute the conventional field growing of ginger for harvesting of high-quality clean gingerols and shogaol with no residue of the pesticides used during the field growing.

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Authors and Affiliations

  1. Centre for Plant Biotechnology and Molecular Biology, Kerala Agricultural University, Thrissur, 680 656, India

    Manjusha Rani, M. R. Shylaja & Deepu Mathew

  2. Directorate of Research, Kerala Agricultural University, Thrissur, 680 656, India

    M. Asha Shankar

  3. Radio Tracer Laboratory, Kerala Agricultural University, Thrissur, 680 656, India

    P. Sureshkumar

  4. All India Coordinated Research Project on Medicinal and Aromatic Plants, Kerala Agricultural University, Thrissur, 680 656, India

    C. Beena

  5. Department of Agricultural Microbiology, College of Agriculture, Kerala Agricultural University, Thrissur, 680 656, India

    D. Girija

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  1. Manjusha Rani
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Correspondence to Deepu Mathew.

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Significance statement: This paper details viable and alternate strategy for the clean synthesis of industrially important gingerols and shogaol. The protocols and varietal, explant and maturity factors, for the maximum in vitro recovery of these molecules, are discussed.

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Rani, M., Shylaja, M.R., Mathew, D. et al. Potential of Microrhizomes for In Vitro Gingerol and Shogaol Synthesis in Ginger (Zingiber officinale Rosc.). Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 92, 121–129 (2022). https://doi.org/10.1007/s40011-021-01314-2

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