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Molecular Detection and Assessment of Hemato-Biochemistry, Oxidant/Antioxidant Status in Natural Canine Monocytic Ehrlichiosis Cases from Northern India

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

The present study was aimed at detecting natural cases of canine monocytic ehrlichiosis (CME) by molecular and cytological methods and assessment of clinico-patho-biochemical parameters among the affected population. Nested polymerase chain reaction was found to be highly sensitive (47.7 %) in detecting the acute cases, followed by buffy coat (29.5 %) and blood smear examination (22.7 %). CME incidence rate was found to be 8.4 %. Hemogram revealed significant (P < 0.01) depletion in the levels of hemoglobin, hematocrit, total erythrocyte count, monocyte and platelet count in diseased dogs as compared to healthy controls. Plasma biochemistry revealed hypoproteinemia, hypoalbuminemia and hyperglobulinemia along with significantly (P < 0.05) higher blood urea nitrogen and alanine aminotransferase values. CME revealed non-significant increase in the levels of erythrocytic lipid peroxides, reduced glutathione and activity of superoxide dismutase, whereas activities of glutathione-S-transferase and glutathione reductase showed significant (P < 0.05) increase and decrease, respectively as compared to healthy controls. Plasma levels of cortisol, insulin and blood level of copper showed non-significant (P > 0.05) reduction, whereas blood level of zinc showed significant (P < 0.05) reduction as compared to healthy controls. These findings revealed that changes in erythrocytic oxidant–antioxidant profile and blood mineral concentration in canine monocytic ehrlichiosis are largely non-significant and inconsistent. Hence, further study is required to elucidate their role in pathogenesis of canine monocytic ehrlichiosis.

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Acknowledgments

The authors would like to thank authorities of Indian Veterinary Research Institute (ICAR), Izatnagar, Bareilly, Uttar Pradesh for providing basic infrastructure and fund to undertake the study.

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Authors and Affiliations

  1. Department of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry (CAU), Selesih, Aizawl, 796 014, Mizoram, India

    Suvendu Kumar Behera

  2. Division of Veterinary Medicine, Indian Veterinary Research Institute, Izatnagar, 243 122, U.P., India

    Umesh Dimri

  3. Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, 243 122, U.P., India

    Parthasarathi Banerjee & Rajat Garg

  4. Indian Veterinary Research Institute, Izatnagar, 243 122, U.P., India

    Satyabrata Dandapat & Bhaskar Sharma

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  1. Suvendu Kumar Behera
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Correspondence to Suvendu Kumar Behera.

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Behera, S.K., Dimri, U., Banerjee, P. et al. Molecular Detection and Assessment of Hemato-Biochemistry, Oxidant/Antioxidant Status in Natural Canine Monocytic Ehrlichiosis Cases from Northern India. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 87, 361–368 (2017). https://doi.org/10.1007/s40011-015-0605-y

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  • DOI: https://doi.org/10.1007/s40011-015-0605-y

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