Abstract
Pulses are high value crops and generally greater attention is paid to protect them against diseases caused by microbial pathogens after harvest during storage. Use of fungicides may cause environmental hazards besides residual problems. Therefore Trichoderma viride, Trichoderma harzianum, Trichoderma virens, Pseudomonas fluorescence and Bacillus subtilis were evaluated in vitro against seed borne diseases of mungbean seeds. In vitro studies were carried out in both dual culture technique and Agar plate methods. The four dominant fungi of mungbean seeds viz, Aspergillus niger, Penicillium rubrum, Alternaria alternata and Fusarium moniliforme were used to assess their vulnerability to an array of bio-agents. Results showed that all bioagents significantly reduced the percent growth inhibition of all tested fungi. Among all the bioagents T. harzianum was found superior in controlling the seed borne mycoflora of mungbean seeds. Among the fungal pathogens, P. rubrum and A. niger were effectively suppressed by T. harzianum and P. fluorescence. Bacillus subtilis, T. virens and T. viride gave the promising results against all the tested fungi. Trichoderma harzianum showed highest inhibition of mycelium growth of the A. alternata followed by T. viride (8.00 %). Bacterial antagonist, B. subtilis showed intermediate effect against all tested fungi. Trichoderma virens and T. viride showed least effect against all tested seed borne fungi.
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Sathyamoorthi K, Amanullah MM, Somasundaram E, Vaiyapuri K (2008) Yield attributes and yield of green gram (Vigna radiata (L.) wilczek) as influenced by increased plant density and nutrient management. Int J Agric Sci 4:719–724
Bakr MA, Rahman ML (1998) Current status of research on mungbean and black gram diseases and future needs. Proceedings of the workshop on diseases resistance breeding in pulse. Bangladesh J Agric Res 11:64–69
Kumar R, Mishra AK, Dubey NK, Tripathi YB (2007) Evaluation of Chenopodium ambrosioides oil as a potential source of antifungal, antiaflatoxigenic and antioxidant activity. Int J Food Microbiol 115:159–164
Tivoli B, Banniza S (2007) Comparison of the epidemiology of Ascochyta blights on grain legumes. Eur J Plant Pathol 119:59–76
Mukhopadhyay AN, Shrestha SM, Mukherjee PK (1992) Biological seed treatment for control of soil born plant pathogens. FAO Plant Prot Bull 40:221–230
Raguchander T, Rajappan K, Samiappan R (1997) Evaluating methods of application of biocontrol agents in the control of Mungbean root rot. Ind Phytopathol 50:229–234
Sankar P, Jeyarajan R (1996) Seed treatment for biological control of Macrophomina phaseolina in sesamum. Ind Phytopathol 49:148–151
Bailey B, Bae H, Strem MD, Crozier J, Thomas SE, Samuels GJ, Vinyard B, Holmes KA (2008) Antibiosis, mycoparasitism and colonization success for endophytic Trichoderma isolates with biological control potential in Theobroma cacao. Biol Control 46:24–35
Hassanein AM, El-Garhy AM, Mekhemar GA (2006) Symbiotic nitrogen fixation process in faba bean and chickpea as affected by biological and chemical control of root-rot. J Agric Sci Mansoura Univ 31(2):963–980
Siddiqui ZA, Akhtar MS (2007) Biocontrol of a chickpea root rot disease complex with phosphate solubilizing microorganisms. J Plant Pathol 9(1):67–77
Pal K, Gardener BM (2006) Biological control of plant pathogens. Plant Health Instr. doi:10.1094/PHI-A-2006-1117-02.P1-25
Khetan SK (2001) Microbial pest control. Marcel Dekker, Inc, New York, p 300
Wang H, Ng TB (2004) Eryngin, a novel antifungal peptide from bodies of the edible mushroom Pleurotus eryngii. Peptides 25:1–5
Chittihunsa T, Bangeekhan E, Wongsamitkul N, Subsomboon T (2007) Screening of Bacillus sp. suppressing the infection of Trichoderma sp. in mushroom cultivation. KMITL Sci Technol J 7:19–27
Shanmugam U, Rajuchandur T, Ramiyappan R (2003) Management of groundnut root rot disease caused by Macrophomina phaseolina with Pseudomonas fluorescens. Ann Plant Prot Sci 11:304–308
Cook RJ (1985) Biological control of plant pathogens: theory application. Phytopathology 75:25–29
Muthamilan M, Jeyarajan R (1996) Integrated management of Sclerotium root rot of groundnut involving T. harzianum, Rhizobium and carbendazim. Indian J Mycol Plant Pathol 16:29–39
Chen C, Belanger RR, Benhamou N, Paulitz TZ (2000) Defense enzymes induced in cucumber roots by treatments with plant growth promoting rhizobacteria (PGPR) and Pythium aphanidermatum. Physiol Mol Plant Pathol 56:13–23
Chang IP, Kommedahl T (1986) Biological control of seedling blight of corn by coating kernels with antagonistic microorganisms. Phytopathol 58:1395–1401
Kommedahl T, Chang IP (1975) Biocontrol of corn root infection in the field by seed treatment with antagonists. Phytopathol 65:296–300
Denis C, Webster J (1971) Antagonistic properties of species groups of Trichoderma. I. Production of non-volatile antibiotics. Trans Br Mycol Soc 57:25–39
Vincent SM (1947) Distortion of fungal hyphae in the presence of certain inhibitors. Nature 150:850–876
Ashwini C, Giri GK (2014) Control of seed borne fungi in green gram and black gram through bioagents. IJABPT 5:3–7
Raghuchandar T, Rajappan K, Samippan R (1997) Evaluating methods of application of biocontrol agent in the control of mungbean root rot. Ind Phytopathol 50(2):229–234
Singh S, Sinha A, Mishra J (2014) Evaluation of different treatment on the occurrences of seed borne fungi of mungbean seed. Afr J Agric Res 9:3301–3303
Sukanta D, Raj SK (1998) Biological control of collar rot (Aspergillus niger) of groundnut. J Oilseed Res 15:334–338
Mogle UP, Maske SR (2009) Efficacy of bioagents and fungicides on seed mycoflora, germination and vigour index of cowpea. Curr Bot 5:06–07
Ram GD, Chauhan SS, Verma DK (2012) In vitro propagation of Jatropha curcas from embryo and nodal explants. Bioscan 7:251–254
Rathod LR, Jadhav MD, Mane SK, Muley SM, Deshmukh PS (2012) Seed borne mycoflora of legume seeds. Int J Adv Biotechnol Res 3:530–532
Rajeswari B, Kumari KVSM (2009) Bioagents and fungicides for the management of seed and seedling diseases of soybean. Indian J Plant Protect 37:127–131
Ghosh C, Pawar NB, Kshirsagar CR, Jadhav AC (2002) Studies on management of leaf spot caused by Alternaria alternata on gerbera. J Maharashtra Agric Univ 27:165–167
Basha ST, Suvarna JT, Hemalatha M, Reddy NPE (2010) Compatibility of native potential bioagents with different fungicides against Colletotrichum gloeosporioides causing Mango Anthracnose. Bioscan 5:19–20
Vadivel S, Ebenezar EG (2006) Eco-friendly management of leaf blight of tomato caused by Alternaria solani. J Mycol Plant Pathol 36(1):79–83
Gawade SB, Mandhare VK, Padule DN, Chavan (2010) Efficacy of bioagents on seed mycoflora, seed germination, seedling vigor index and field emergence in sunflower. PKV Res J 34:65–69
Lo CT, Nelson EB, Harman GE (1997) Improved bio control efficacy of Trichoderma harzianum 1295-22 for foliar phases of turf diseases by use of spray applications. Plant Dis 81(10):1132–1138
Leeman M, van Pelt JA, Hendrickx MJ, Scheffer RJ, Bakker PAHM, Schippers B (1995) Biocontrol of Fusarium wilt of radish in commercial greenhouse trials by seed treatment with Pseudomonas fluorescens WCS374. Phytopathol 85:1301–1305
D’Ercole N, Nipoti P, Finessi LE, Manzali D (1988) Review of several years of research in Italy on the biological control of soil fungi with Trichoderma spp. Bull OEPP 18:95–102
Dubeikovsky AN, Mordukhova EA, Kochethov VV, Polikarpova RY, Boronin AM (1993) Growth promotion of blackcurrant softwood cuttings by recombinant strain Pseudomonas florescence BSP53a synthesizing an increased amount of indole-3-acetic acid. Soil Biol Biochem 25:1277–1281
Kloepper JW, Lifshittz R, Zablotowicz RM (1989) Free-living bacterial inocula for enhancing crop productivity. Trends Biotechnol 7:39–44
Davison J (1988) Plant beneficial bacteria. Biotechnology 6:282–286
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The authors wish to acknowledge the Head, Dr. Shewta Srivastava, Department of Mycology and Plant Pathology, Institute of Agricultural Sciences B.H.U Varanasi for providing necessary laboratory facilities.
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Singh, S., Sinha, A., Yadav, S.M. et al. Antagonistic Behavior of Different Bioagents Against Dominant Seed Borne Fungi of Mungbean Seeds Under In Vitro Condition. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 87, 599–602 (2017). https://doi.org/10.1007/s40011-015-0604-z
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DOI: https://doi.org/10.1007/s40011-015-0604-z