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Influence of Solvent Types and Source of Collection on Total Phenolic Content and Antioxidant Activities of Acorus calamus L.

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Abstract

Acorus calamus (Family Araceae) is an important medicinal plant of Himalayan region largely used in the preparation of cosmetics and traditional medicines. Total phenolic content and antioxidant activity varied among the populations and solvent system. Solvents with medium polarity such as acetone and methanol were found best for extraction of total phenolic content and antioxidant activity. Total phenolic content ranged between 4.92 mg gallic acid equivalent (GAE)/g dry weight (dw) in distilled water to 8.09 mg GAE/g dw in acetone extract among different populations. Irrespective of the solvents, Jyoli population showed the maximum phenolic content (8.86 mg GAE/g dw), however, Someshwar population was found best (9.97 mg GAE/g dw) in acetone extract. Antioxidant activity measured by three in vitro assay, i.e., 2, 2′- azinobis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical scavenging, 1,1-Diphenyl-2-picryhydrazyl (DPPH) radical scavenging and ferric reducing antioxidant power (FRAP) assay showed a significant variation (p < 0.01) among populations. Jyoli population was found best for antioxidant activity (ABTS 13.68 mM; DPPH 6.79 mM; and FRAP 5.14 mM ascorbic acid equivalent/100 g dw). Total phenolic content showed a significant positive relationship with antioxidant activity. The results of the present study are indicative that the species can be a good source of natural antioxidant.

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Authors and Affiliations

  1. G. B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, 263 643, Uttarakhand, India

    Amit Bahukhandi, Sandeep Rawat, Indra D. Bhatt & Ranbeer S. Rawal

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  1. Amit Bahukhandi
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  2. Sandeep Rawat
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  3. Indra D. Bhatt
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  4. Ranbeer S. Rawal
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Correspondence to Indra D. Bhatt.

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Bahukhandi, A., Rawat, S., Bhatt, I.D. et al. Influence of Solvent Types and Source of Collection on Total Phenolic Content and Antioxidant Activities of Acorus calamus L.. Natl. Acad. Sci. Lett. 36, 93–99 (2013). https://doi.org/10.1007/s40009-012-0109-8

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  • DOI: https://doi.org/10.1007/s40009-012-0109-8

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