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Design and synthesis of potential nano-carrier for delivery of diphencyprone to hair follicle

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Abstract

Purpose

Poor bioavailability of diphenylcyclopropenone (DPCP), an effective drug for an autoimmune disorder of alopecia areata, limits its pharmaceutical effects. Carriers-based nanoparticles with especially porous structures can overcome these restrictions. Here, mesoporous silica nanoparticles, MCM41, with high surface area and pore volume were synthesized for DPCP delivery to the hair follicles through skin tissue.

Methods

Mesoporous silica nanoparticles (MCM41) were synthesized by the Stöber process and analyzed by scanning electron microscopy, dynamic light scattering, Barrett–Joiner–Halenda and N2 adsorption isotherms for their physicochemical properties. HPLC method was used to determine drug entrapment efficiency and release behavior during 24 h. Cytotoxicity of formulations was evaluated using MTT assay and permeation profiles of DPCP (control sample) and DPCP-MCM41 into the rat skin were obtained by using Franz diffusion cells. Fluorescence microscopy and intrafollicular nanoparticle accumulation were examined using confocal microscopy.

Results

Experiments showed the spherical shape of the nanoparticles with an average size of 50 ± 3 nm, high surface area, and porosity. Entrapment efficiency was about 90% and release behavior had sustained manner after 3 h. Fluorescent and confocal microscopy confirmed that the nanoparticles passed through follicular channels and aggregated around the hair follicles.

Conclusion

MCM41 nanoparticles provide a promising nano-carrier for targeted drug delivery of DPCP to the human hair follicle.

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Authors and Affiliations

  1. Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Islamic Republic of Iran

    Salar Hooshyar & Bita Mehravi

  2. Department of Medical Nanotechnology, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Islamic Republic of Iran

    Salar Hooshyar & Bita Mehravi

  3. Department of Dermatology, University of California, San Francisco, CA, USA

    Shohreh Nafisi

  4. Department of Chemistry, Central Tehran Branch, Islamic Azad University, Tehran, Islamic Republic of Iran

    Shohreh Nafisi

  5. Radiation Biology Research Center, Iran University of Medical Sciences, Tehran, 1449614535, Islamic Republic of Iran

    Mojdeh Mohseni & Bita Mehravi

Authors
  1. Salar Hooshyar
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  2. Shohreh Nafisi
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  3. Mojdeh Mohseni
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  4. Bita Mehravi
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Corresponding author

Correspondence to Bita Mehravi.

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The authors declare that they have no conflict of interest.

Statement of human and animal rights

The animal studies were performed after receiving approval of the Institutional Animal Care and Use Committee in the Iran University of Medical Science (IACUC approval No. IR.IUMS.REC 1396.28678).

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Hooshyar, S., Nafisi, S., Mohseni, M. et al. Design and synthesis of potential nano-carrier for delivery of diphencyprone to hair follicle. J. Pharm. Investig. 51, 173–181 (2021). https://doi.org/10.1007/s40005-020-00501-z

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  • DOI: https://doi.org/10.1007/s40005-020-00501-z

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