Abstract
Anomala dimidiata, a key white grub species associated with substantial crop losses in Indian Himalayan region, belong to one of the largest genera under scarab tribe, Anomalini. This paper presents the molecular variation and phylogenetic relationship of this species from the region using partial cytochrome oxidase I (COI) and cytochrome b (cyt b) sequences of mitochondrial genome in both nucleotides and polypeptides. The COI sequences were compared with available five sequences of A. dimidiata and 12 sequences of species under genus Anomala to study the divergence within and between species, respectively. However, cyt b sequence, as we were presenting for the first time, was compared with cyt b sequences of Drosophila yakuba whose entire mitochondrial genome was known and Dasylepida ishigakiensis, a scarabid white grub species, whose partial cyt b sequence is available. Both the genes were A + T biased and showed differential codon usage between species and geographical isolates. This is evidenced by discrepancies between nucleotide and deduced polypeptide sequences. The phylogenetic relationship of COI sequences also revealed that A. dimidata was evolutionarily associated with A. xanthoptera. The evolutionary distances of three cyt b sequences showed that differences were larger than expected based on evolutionary divergence. The study illustrates a complex genetic variation coupled with highly structured evolutionary divergences between and within species of Anomala.
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Ahrens D, Monaghan MT, Vogler AP (2007) DNA-based taxonomy for associating adults and larvae in multi-species assemblages of chafers (Coleoptera: Scarabaeidae). Mol Phylogenet Evol 44:436–449
Avise JC (1994) Molecular markers, natural history and evolution. Chapman & Hall, New York
Brower VZ (1996) Parallel race7 formation and the evolution of mimicry in Heliconius butterflies, a phylogenetic hypothesis from mitochondrial DNA sequences. Evolution 50:195–221
Caterino MS, Cho S, Sperling FAH (2000) The current state of insect molecular systematics: a thriving tower of babel. Ann Rev Entomol 45:1–54
Collins AM, Gardner LM (2001) Partial cytochrome b sequences for six Hymenoptera of the Eastern United States. J Hered 92:519–521
Dittrich G, Conlong DE, Mitchelle A (2006) Molecular identification of South African sugar cane white grubs (Coleopetra: Scarabaeidae). Proc S Afr Sug Technol Ass 80:264–268
Funk DJ (1999) Molecular systematics of cytochrome oxidase I and 16S from Neochlamisus leaf beetles and the importance of sampling. Mol Biol Evol 16:67–82
Gasteiger E, Gattiker A, Hoogland C, Ivanyi I, Appel RD, Bairoch A (2003) ExPASy: the proteomics server for in-depth protein knowledge and analysis. Nucleic Acids Res 31:3784–3788
Havill NP, Foottit RG, Von Dohlen CD (2007) Evolution of host specialization in the Adelgidae (Insecta: Hemiptera) inferred from molecular phylogenetics. Mol Phylogenet Evol 44:357–370
Hebert PDN, Cywinska A, Ball SL, deWaard JR (2003) Biological identifications through DNA barcodes. Proc R Soc B Biol Sci 270:313–321
Henry JM, Raina AK, Ridgway RL (1990) Isolation of high-molecular-weight DNA from insects. Anal Biochem 185:147–150
Howland DE, Hewitt GM (1995) Phylogeny of Coleoptera based on mitochondrial cytochrome oxidase I sequence data. Insect Mol Biol 4:203–215
Jameson ML, Paucar-Cabrera A, Solis A (2003) Synopsis of the new world genera of Anomalini (Coleoptera: Scarabaeidae: Rutelinae) and description of a new genus from Costa Rica and Nicaragua. Ann Entomol Soc Am 96:415–432
Jermiin LS, Crozier RH (1994) The cytochrome b region in the mitochondrial DNA of the ant, Tetraponera rufoniger: sequence divergence in hymenoptera may be associated with nucleotide content. J Mol Evol 38:282–294
Karimi J, Darsoei R, Fattah-Hosseini F, Sadeghi H (2012) Phylogenetic analysis on some Iranian white grubs with new data about natural pathogen of Polyphylla adspersa. J Entomol Res Soc 14:1–13
Kranthi S, Kranthi KR, Bharose AA, Syed SN, Dhawad CS, Wadaskar RM, Behere GT, Patil EK (2006) Cytochrome oxidase I sequence of Helicoverpa (Noctuidae: Lepidoptera) species in India—its utility as a molecular tool. Indian J Biotechnol 5:195–199
Lunt DH, Zhang DX, Szymura JM, Hewitt GM (1996) The insect cytochrome oxidase I gene: evolutionary patterns and conserved primers for phylogenetic studies. Insect Mol Biol 5:153–165
McWilliam H, Li W, Uludag M, Squizzato S, Park YM, Buso N, Cowley AP, Lopez R (2013) Analysis tool web services from the EMBL-EBI. Nucleic Acids Res. doi:10.1093/nar/gkt376
Mestrovic N, Mravinac B, Plohl M, Ugarkovic D, Bruvo-Mađaric B (2006) Preliminary phylogeny of Tribolium beetles (Coleoptera: Tenebrionidae) resolved by combined analysis of mitochondrial genes. Eur J Entomol 103:709–715
Mico E, Piau O, Galante E, Lumaret JP (2003) Taxonomy of Iberian Hoplia (Coleoptera, Scarabaeoidea, Hopliinae) based on mtDNA analysis. Mol Phylogenet Evol 26:348–353
Muraji M, Nakahara S (2001) Phylogenetic relationships among fruit flies, Bactrocera (Diptera, Tephritidae), based on the mitochondrial rDNA sequences. Insect Mol Biol 10:549–559
Muraji M, Kawasaki K, Shimizu T (2000) Phylogenetic utility of nucleotide sequences of mitochondrial 16S ribosomal RNA and cytochrome b genes in anthocorid bugs (Heteroptera: Anthocordiae). Appl Entomol Zool 35:293–300
Muraji M, Kawasaki K, Shimizu T (2001) Nucleotide sequence variation and use of mitochondrial DNA for phylogenetic analyses in Anthocorid bugs (Hemiptera: Anthocoridae). Jpn Agric Res Q 35:85–90
Muraji M, Hirai Y, Akion T, Wakamura S, Arakaki N (2008) Genetic divergence among populations of the white grub beetles, Dasylepida ishigakiensis (Coleoptera: Scarabaeidae), distributed in the southern part of the Ryukyu island of Japan, detected from mitochondrial DNA sequences. Appl Entomol Zool 43:287–292
Page TJ, Choy SC, Hughes JM (2005) The taxonomic feedback loop: symbiosis of morphology and molecules. Biol Lett 1:139–142
Pons J, Barraclough T, Theodorides K, Cardoso A, Vogler A (2004) Using exon and intron sequences of the gene Mp20 to resolve basal relationships in Cicindela (Coleoptera: Cicindelidae). Syst Biol 53:554–570
Rao S, Liston A, Crampton L, Takeyasu J (2006) Identification of larvae of exotic Tipulapaludosa (Diptera: Tipulidae) and Toleracea in North America using mitochondrial cytB sequences. Ann Entomol Soc Amer 99:33–40
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
Simmons RB, Weller SJ (2001) Utility and evolution of cytochrome b in insects. Mol Phylogenet Evol 20:196–210
Sperling FA, Hickey D (1994) Amplified mitochondrial DNA as a diagnostic marker for species of conifer-feeding Choristoneura. Can Entomol 127:277–288
Sushil SN, Mohan M, Selvakumar G, Bhatt JC (2006) Relative abundance and host preference of white grubs (Coleoptera: Scarabaeidae) in Kumaon hills of Indian Himalayas. Indian J Agric Sci 76:338–339
Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA 4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599
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Authors are thankful to Indian Council of Agricultural Research (ICAR), New Delhi, for funding the study.
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Subbanna, A.R.N.S., Kalyana babu, B., Stanley, J. et al. Phylogeny and Genetic Divergence of Indian Himalayan Population of Anomala dimidiata (Coleoptera: Scarabaeidae) Inferred from Mitochondrial DNA Sequences. Agric Res 5, 64–71 (2016). https://doi.org/10.1007/s40003-015-0194-2
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DOI: https://doi.org/10.1007/s40003-015-0194-2